{Reference Type}: Journal Article
{Title}: Complete-genome sequence and in vitro probiotic characteristics analysis of Bifidobacterium pseudolongum YY-26.
{Author}: Yu Y;Ren X;Cao L;Liang Q;Xiao M;Cheng J;Nan S;Zhu C;Kong Q;Fu X;Mou H;Yu Y;Ren X;Cao L;Liang Q;Xiao M;Cheng J;Nan S;Zhu C;Kong Q;Fu X;Mou H;
{Journal}: J Appl Microbiol
{Volume}: 133
{Issue}: 4
{Year}: Oct 2022
{Factor}: 4.059
{DOI}: 10.1111/jam.15730
{Abstract}: <B>OBJECTIVE: </B>The aim was to isolate a neotype bifidobacteria strain and evaluate its in vitro probiotic potential.<BR><B>RESULTS: </B>Bifidobacterium pseudolongum YY-26 (CGMCC 24310) was isolated from faeces of mice treated with low-molecular-weight hydrolyzed guar gum (GMPS) and identified based on 16S rRNA sequence and genome sequence. Whole-genome sequencing obtained using PacBio's single-molecular and Illumina's paired-end sequencing technology. A genome of 2.1 Mb in length, with 1877 predicted protein-coding sequences was obtained. Carbohydrate-Activity enZyme analysis revealed that YY-26 encodes 66 enzymes related to carbohydrate metabolism. Whole genome sequence analysis revealed the typical probiotic characteristics of YY-26, including safety in genetic level and ability to produce beneficial metabolites and extracellular polysaccharides. Ability of extensive carbon source utilization and short-chain fatty acid production was observed with single YY-26 cultivation. Considerable acetic acids and lactic acids were determined in GMPS utilization. YY-26 showed tolerance to simulated gastrointestinal tract and displayed appreciable antioxidant activity of free radical scavenging.<BR><B>CONCLUSIONS: </B>B. pseudolongum YY-26 was identified with numerous probiotic-associated genes and its probiotic characteristics were verified in vitro.<BR><B>CONCLUSIONS: </B>This study supplemented with limited publicly information regarding the genomes of B. pseudolongum strains and revealed the probiotic potential of YY-26.