■根据微环境的不同,γδT细胞可能具有与Th1,Th2,Th17,调节性T细胞或抗原呈递细胞相似的特征。尽管Th1/Th2平衡对妊娠相关疟疾和结局的影响有广泛的文献记载,目前尚无关于γδT细胞表型改变与胎盘疟疾(PM)与妊娠结局之间关系的报道。本研究旨在研究γδT细胞及其亚群在胎盘恶性疟原虫疟疾中的参与。
■在雅温得进行的一项病例对照研究中,喀麦隆从2022年3月到2023年5月,外围,在分娩时从50名妇女收集胎盘和脐带血样品(如通过光学显微镜诊断的,29PM阴性:PM-和21PM阳性:PM+)。使用血红蛋白计测量血红蛋白水平。PBMC,使用hisopaque-1077分离IVBMC和CBMC,并用于表征总γδT细胞群体和亚群(Vδ1,Vδ2+,Vδ1-Vδ2-)通过流式细胞术。
■胎盘恶性疟原虫感染与IVBMC中总γδT细胞和PBMC和IVBMC中Vδ1亚群的频率显着增加有关,但PBMC和IVBMC中Vδ2+亚群的频率降低。激活标记的表达:HLA-DR,与PM-组相比,PM中总γδT细胞和亚群中的耗竭标志物(PD1和TIM3)显着上调。IVBMC中总γδT细胞的频率,IVBMC中总γδT细胞和亚群的TIM-3表达,总γδT细胞内的HLA-DR表达和IVBMC中的Vδ2+亚群与母体血红蛋白水平呈负相关。此外,PBMC中总γδT细胞的频率和CBMC中Vδ2+亚群中PD1的表达与出生体重呈负相关,这与PBMC中Vδ1-Vδ2-亚群的频率和IVBMC中Vδ2+亚群中HLA-DR的表达呈正相关,与母体血红蛋白水平和出生体重呈正相关,分别。
■数据表明恶性疟原虫胎盘疟疾中激活和耗尽的γδT细胞上调,对妊娠结局的影响,包括母亲血红蛋白水平和出生体重。
UNASSIGNED: Depending on the microenvironment, γδ T cells may assume characteristics similar to those of Th1, Th2, Th17, regulatory T cells or antigen presenting cells. Despite the wide documentation of the effect of Th1/Th2 balance on pregnancy associated malaria and outcomes, there are no reports on the relationship between γδ T cell phenotype change and Placental Malaria (PM) with pregnancy outcomes. This study sought to investigate the involvement of γδ T cells and its subsets in placental Plasmodium falciparum malaria.
UNASSIGNED: In a case-control study conducted in Yaoundé, Cameroon from March 2022 to May 2023, peripheral, placental and cord blood samples were collected from 50 women at delivery (29 PM negative: PM- and 21 PM positive: PM+; as diagnosed by light microscopy). Hemoglobin levels were measured using hemoglobinometer. PBMCs, IVBMCs and CBMCs were isolated using histopaque-1077 and used to characterize total γδ T cell populations and subsets (Vδ1+, Vδ2+, Vδ1-Vδ2-) by flow cytometry.
UNASSIGNED: Placental Plasmodium falciparum infection was associated with significant increase in the frequency of total γδ T cells in IVBMC and of the Vδ1+ subset in PBMC and IVBMC, but decreased frequency of the Vδ2+ subset in PBMC and IVBMC. The expression of the activation marker: HLA-DR, and the exhaustion markers (PD1 and TIM3) within total γδ T cells and subsets were significantly up-regulated in PM+ compared to PM- group. The frequency of total γδ T cells in IVBMC, TIM-3 expression within total γδ T cells and subsets in IVBMC, as well as HLA-DR expression within total γδ T cells and Vδ2+ subset in IVBMC were negatively associated with maternal hemoglobin levels. Furthermore, the frequency of total γδ T cells in PBMC and PD1 expression within the Vδ2+ subset in CBMC were negatively associated with birth weight contrary to the frequency of Vδ1-Vδ2- subset in PBMC and HLA-DR expression within the Vδ2+ subset in IVBMC which positively associated with maternal hemoglobin level and birth weight, respectively.
UNASSIGNED: The data indicate up-regulation of activated and exhausted γδ T cells in Plasmodium falciparum placental malaria, with effects on pregnancy outcomes including maternal hemoglobin level and birth weight.