这项研究调查了细菌环状脂肽(LP;表面活性蛋白,iturins,fengycins)对微生物相互作用的影响。目的是研究细菌的存在是否抑制真菌生长,以及这种抑制是否是由于细菌代谢产物的释放,尤其是LP。在尖孢镰刀菌存在下培养具有已知植物生长促进潜力的选定内生细菌菌株。strigae(Fos),被用作模型真菌生物。测试细菌的细胞外代谢组,专注于LP,特点是,研究了细菌LP对真菌生长的抑制作用。结果表明,维氏芽孢杆菌GB03和FZB42以及枯草芽孢杆菌BSn5对Fos的拮抗作用最强。ParaburkholderiaphyfirmansPsJN,另一方面,倾向于有轻微的,虽然没有显著的增长促进作用。菌株GB03和FZB42的粗LP对Fos的抑制作用最强,具有显著抑制孢子萌发和菌丝结构的破坏作用。液相色谱串联质谱显示了几种伊杜林变体的产生,芬霉素,和表面活性蛋白LP家族来自菌株GB03,FZB42和BSn5,具有不同的强度。使用平板培养,在存在Fos的情况下,在菌株GB03,FZB42和BSn5中检测到较高的杆菌霉素D组分。此外,双板培养物中Fos的存在引发了芽孢杆菌菌株中杆菌霉素D产量的增加。这项研究证明了某些芽孢杆菌菌株的有效拮抗作用(即,GB03、FZB42、BSn5)对Fos发展。我们的发现强调了微生物相互作用在塑造微生物组合共存中的关键作用。
This study investigated the influence of bacterial cyclic lipopeptides (LP; surfactins, iturins, fengycins) on microbial interactions. The objective was to investigate whether the presence of bacteria inhibits fungal growth and whether this inhibition is due to the release of bacterial metabolites, particularly LP. Selected endophytic bacterial strains with known plant-growth promoting potential were cultured in the presence of Fusarium oxysporum f.sp. strigae (Fos), which was applied as model fungal organism. The extracellular metabolome of tested bacteria, with a focus on LP, was characterized, and the inhibitory effect of bacterial LP on fungal growth was investigated. The results showed that Bacillus velezensis GB03 and FZB42, as well as B. subtilis BSn5 exhibited the strongest antagonism against Fos. Paraburkholderia phytofirmans PsJN, on the other hand, tended to have a slight, though non-significant growth promotion effect. Crude LP from strains GB03 and FZB42 had the strongest inhibitory effect on Fos, with a significant inhibition of spore germination and damage of the hyphal structure. Liquid chromatography tandem mass spectrometry revealed the production of several variants of iturin, fengycin, and surfactin LP families from strains GB03, FZB42, and BSn5, with varying intensity. Using plate cultures, bacillomycin D fractions were detected in higher abundance in strains GB03, FZB42, and BSn5 in the presence of Fos. Additionally, the presence of Fos in dual plate culture triggered an increase in bacillomycin D production from the Bacillus strains. The study demonstrated the potent antagonistic effect of certain Bacillus strains (i.e., GB03, FZB42, BSn5) on Fos development. Our findings emphasize the crucial role of microbial interactions in shaping the co-existence of microbial assemblages.