PDF(Pubmed)
Abstract：
Myeloblastosis (MYB)-like proteins are a family of highly conserved transcription factors in animals, plants, and fungi and are involved in the regulation of mRNA expression of genes. In this study, we identified and characterized one MYB-like protein in the model organism Aspergillus nidulans. We screened the mRNA levels of genes encoding MYB-like proteins containing two MYB repeats in conidia and found that the mRNA levels of four genes including flbD, cicD, and two uncharacterized genes, were high in conidia. To investigate the roles of two uncharacterized genes, AN4618 and AN10944, deletion mutants for each gene were generated. Our results revealed that AN4618 was required for fungal development. Therefore, we further investigated the role of AN4618, named as mylA, encoding the MYB-like protein containing two MYB repeats. Functional studies revealed that MylA was essential for normal fungal growth and development. Phenotypic and transcriptomic analyses demonstrated that deletion of mylA affected stress tolerance, cell wall integrity, and long-term viability in A. nidulans conidia. In addition, the germination rate of the mylA deletion mutant conidia was decreased compared with that of the wild-type conidia. Overall, this study suggests that MylA is critical for appropriate development, conidial maturation, dormancy, and germination in A. nidulans.
摘要：
成髓细胞病(MYB)样蛋白是动物中高度保守的转录因子家族,植物,和真菌，并参与基因mRNA表达的调控。在这项研究中,我们在模型生物构巢曲霉中鉴定并鉴定了一种MYB样蛋白。我们筛选了分生孢子中含有两个MYB重复序列的MYB样蛋白基因的mRNA水平,cicD,和两个没有特征的基因，分生孢子含量很高。为了研究两个未表征基因的作用，AN4618和AN10944,产生每个基因的缺失突变体。我们的结果表明，AN4618是真菌发育所必需的。因此,我们进一步研究了AN4618的作用，名为mylA，编码含有两个MYB重复序列的MYB样蛋白。功能研究表明，MylA对于正常的真菌生长和发育至关重要。表型和转录组分析表明mylA的缺失会影响胁迫耐受性，细胞壁完整性，和长期生存能力在A.nidulans分生孢子。此外,与野生型分生孢子相比，mylA缺失突变分生孢子的发芽率降低。总的来说,这项研究表明MyLA对于适当的发展至关重要，分生孢子成熟，休眠,并在A.nidulans中发芽。
