Abstract：
Beauveria bassiana, the causative agent of arthropod, proliferates in the host hemolymph (liquid environment) and shits to saprotrophic growth on the host cadaver (aerial surface). In this study, we used transcriptomic analysis to compare the gene expression modes between these two growth phases. Of 10,366 total predicted genes in B. bassiana, 10,026 and 9985 genes were expressed in aerial (AM) and submerged (SM) mycelia, respectively, with 9853 genes overlapped. Comparative analysis between two transcriptomes indicated that there were 1041 up-regulated genes in AM library when compared with SM library, and 1995 genes were down-regulated, in particular, there were 7085 genes without significant change in expression between two transcriptomes. Furthermore, of 25 amidase genes (AMD), BbAMD5 has high expression level in both transcriptomes, and its protein product was associated with cell wall in aerial and submerged mycelia. Disruption of BbAMD5 significantly reduced mycelial hydrophobicity, hydrophobin translocation, and conidiation on aerial plate. Functional analysis also indicated that BbAmd5 was involved in B. bassiana blastospore formation in broth, but dispensable for fungal virulence. This study revealed the high similarity in global expression mode between mycelia grown under two cultivation conditions.
摘要：
白僵菌,节肢动物的病原体，在宿主血淋巴（液体环境）中增殖，并在宿主尸体（气生表面）上形成腐生生长。在这项研究中,我们使用转录组学分析来比较这两个生长阶段的基因表达模式。在白僵菌的10366个预测基因中，10,026和9985个基因在空中（AM）和浸没（SM）菌丝体中表达，分别,与9853个基因重叠。两个转录组之间的比较分析表明，与SM文库相比，AM文库中有1041个上调基因。1995年基因下调，特别是,有7085个基因在两个转录组之间的表达没有显着变化。此外，25个酰胺酶基因（AMD），BbAMD5在两个转录组中都有高表达水平，其蛋白产物与气生菌丝体和浸没菌丝体的细胞壁有关。BbAMD5的破坏显着降低了菌丝体的疏水性，疏水蛋白易位,和在空中板块上的分体。功能分析还表明，BbAmd5参与肉汤中的球孢芽孢杆菌囊胚孢子形成，但对真菌毒力来说是可有可无的。这项研究揭示了在两种培养条件下生长的菌丝体之间在整体表达模式上的高度相似性。
