背景:伤寒沙门氏菌是沙门氏菌的特异性菌株,负责引发伤寒;发展中国家的重大公共卫生问题。
目的:本研究旨在鉴定胆囊中的细菌,在患者的胆囊切除术中,通过分离伤寒沙门氏菌并利用微观特征,生化和聚合酶链反应(PCR)测试。
方法:从巴格达教学医院收集了120个标本,伊拉克。从10月开始进行横断面描述性研究,2021年至7月,2022年。在这项研究中,26例(54.2%)男性患者的沙门氏菌检测呈阳性,22例(45.8%)女性患者。患者的年龄从<30岁到>60岁不等。P值>0.05被认为是显著的,以证实患者的年龄与伤寒沙门氏菌效应之间的关系。
结果:在这项研究采集的120个血液样本中,48(40%)通过PCR测试呈阳性,40(33.3%)通过使用Widal测试测试为阳性,35例(29.1%)活检培养阳性,血培养阳性35例(29.1%)。发现所有伤寒沙门氏菌分离株对亚胺培南敏感,头孢吡肟,和头孢曲松,但是对庆大霉素有抗药性,环丙沙星,阿米卡星,红霉素,和四环素(72%,29%,43%,100%,100%,分别)。
结论:实时聚合酶链反应(RT-PCR)测试和Vitek2紧凑系统在伤寒沙门氏菌的检测中显示出很高的准确性。观察到多药耐药性,这应该是减少抗生素消费的信号。
BACKGROUND: Salmonella typhi is a specific strain of the Salmonella bacterium, responsible for triggering typhoid fever; a significant public health concern in developing nations.
OBJECTIVE: The current study aimed to identify the bacteria from the gallbladder, taken during cholecystectomies of patients, by isolating Salmonella typhi and by using microscopic characteristics, biochemical and polymerase chain reaction (PCR) tests.
METHODS: A total of 120 specimens were collected from the Baghdad Teaching Hospital, Iraq. A cross-sectional descriptive study was carried out from October, 2021, to July, 2022. During that study, 26 (54.2%) male patient tested positive for Salmonella typhias well as 22 (45.8%) female patients. The age of the patients varied from < 30 to > 60 years. p-value > 0.05 was considered significant to confirm a relationship between age and Salmonella typhi effect for patients.
RESULTS: Out of the 120 blood samples taken for this study, 48 (40%) tested positive by use of PCR test, 40 (33.3%) tested positive by use of the Widal test, 35 (29.1%) were positive for biopsy culture, and 35 (29.1%) were positive for blood culture. All Salmonella typhi isolates were found to be sensitive to the imipenem, cefepime, and ceftriaxone, but were resistant to gentamycin, ciprofloxacin, amikacin, erythromycin, and tetracycline (72%, 29%, 43%, 100%, 100%, respectively).
CONCLUSIONS: The real time polymerase chain reaction (RT-PCR) tests and the Vitek 2 compact system showed a high level of accuracy in the detection of Salmonella typhi. Multidrug resistance was observed, which should be a signal to reduce antibiotic consumption.