Abstract：
Sauroleishmania spp. comprises one of the four Leishmania subgenera, which has been historically considered a non-pathogenic protozoan of reptiles. However, some strains appear to be transiently infective to mammals, and recent findings have detected these parasites in dogs and humans in areas where leishmaniasis is endemic. Herein, the digestion pattern of PCR-RFLP of the 234 bp-hsp70 fragment was evaluated as a simpler and cheaper tool to distinguish the Sauroleishmania species from the other Leishmania subgenera. As a result, the digestion of the 234 bp-hsp70 fragments with HaeIII produced a banding pattern specific to the four Sauroleishmania strains assessed. This technique could contribute to the identification of Leishmania parasites isolated from sandflies, reptiles, or even mammals in fieldworks as an alternative to the use of laborious and expensive methodologies.
摘要：
Sauroleishmaniaspp.包括四个利什曼原虫亚属之一，历史上被认为是爬行动物的非致病性原生动物。然而,一些菌株似乎对哺乳动物有短暂的感染，最近的发现已经在利什曼病流行地区的狗和人类中发现了这些寄生虫。在这里，234bp-hsp70片段的PCR-RFLP消化模式被评估为一种更简单，更便宜的工具，可以区分Sauroleishmania物种与其他利什曼原虫亚属。因此,用HaeIII消化234bp-hsp70片段产生了对所评估的四种Sauroleishmania菌株具有特异性的条带模式。这项技术可能有助于鉴定从沙蝇中分离出的利什曼原虫寄生虫，爬行动物,甚至是野外工作中的哺乳动物，作为使用费力和昂贵方法的替代方法。
