在科学文献中没有定论的说法,物种Tremamicranthum,广泛分布在巴西各地,可能会产生植物大麻素,可能作为大麻的替代品。在这项研究中,我们进行了一项全面的调查,以评估在巴西中西部地区采集的两份云杉样品中是否存在植物大麻素.在试图检测黑麦草中的大麻素,采用了推荐的大麻筛查测试,固蓝BB盐(FBBBS)比色测定,其次是薄层色谱(TLC)和仪器技术:高效液相色谱耦合二极管阵列检测器(HPLC-DAD)和气相色谱耦合质谱(GC-MS)。当不使用氯仿萃取时,FBBBS试剂对来自黑毛虫所有部位的提取物产生了阳性结果(叶子,分支,水果,和花序)。然而,这些来自FBBBS测试的初步阳性结果,表明大麻素的存在,没有得到FBBBS随后氯仿萃取的证实,TLC,或这项研究中使用的仪器技术。这些额外的结果表明,阳性FBBBS测试结果可能是由于其他酚类化合物而不是植物大麻素的存在。例如,在黑木耳提取物中存在的牡蛎素类化合物可能解释了FBBBS试验结果的阳性。因此,新的断言,T.micranthum生产大麻素将需要更多的选择性实验的支持,以避免基于较少选择性筛选测试的假阳性声明。
There are inconclusive claims in the scientific literature that the species Trema micranthum, widely distributed throughout the Brazilian territory, may produce
phytocannabinoids, potentially serving as an alternative to Cannabis sativa. In this study, we conducted a comprehensive investigation to assess the presence of
phytocannabinoids in two Trema micranthum samples collected in the Midwest region of Brazil. In trying to detect cannabinoids in T. micranthum, a recommended cannabis screening test was employed, the Fast Blue BB Salt (FBBBS) colorimetric assay, followed by thin-layer chromatography (TLC) and instrumental techniques: high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) and gas chromatography coupled to mass spectrometry (GC-MS). When employed without chloroform extraction, the FBBBS reagent yielded positive results for extracts from all parts of T. micranthum (leaves, branches, fruits, and inflorescences). However, these initial positive results from the FBBBS test, suggesting the presence of cannabinoids, were not corroborated by FBBBS followed by chloroform extraction, TLC, or the instrumental techniques used in this study. These additional outcomes suggest that the positive FBBBS test results were likely due to the presence of other phenolic compounds rather than
phytocannabinoids. For example, the presence of vitexin-like compounds in T. micranthum extracts might explain the positive FBBBS test results. Therefore, new assertions that T. micranthum produces cannabinoids will require the support of more selective experiments to avoid false-positive claims based on less selective screening tests.