Abstract：
In this study, we developed and validated a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of eight phytocannabinoids in various cannabidiol (CBD) products from Japanese market. This method was combined with electrospray ionization in positive mode and sample preparation with QuEChERS. Three types of commercial products such as honey, chocolate, and gummies were used to perform accurate quantification with unified protocol of LC-MS/MS and QuEChERS. The limit of detection and quantification were 5-20 µg g-1 and 10-40 µg g-1, respectively. Reproducibility was ensured using matrices free of target foods, resulting in an accuracy within ±10 % and a precision with a relative standard deviation of less than 5 % for all targets. Finally, this analytical method was applied to 8 series of commercial samples from the Japanese market. This unified protocol will serve as a reference as an official method in Japan.
摘要：
在这项研究中,我们开发并验证了液相色谱串联质谱（LC-MS/MS）方法，用于同时测定来自日本市场的各种大麻二酚（CBD）产品中的八种植物大麻素。该方法与正模式的电喷雾电离和使用QuEChERS的样品制备相结合。三种类型的商业产品，如蜂蜜，巧克力,和软糖用于通过LC-MS/MS和QuEChERS的统一方案进行准确定量。检测限和定量限分别为5-20µgg-1和10-40µgg-1。使用不含目标食物的基质确保了可重复性，对于所有目标，精度在±10％以内，相对标准偏差小于5％的精度。最后,该分析方法适用于来自日本市场的8系列商业样品。该统一协议将作为日本官方方法的参考。
