背景:多目标同时检测的重要性在于其显着提高检测效率的能力,使其成为快速和具有成本效益的测试的宝贵。光电化学(PEC)传感器已成为检测有害物质和生物标志物的有希望的候选者,归因于他们无与伦比的敏感度,最小背景信号,成本效益,设备简单,和出色的重复性。然而,在PEC传感领域,设计一种有效的多目标检测策略仍然是一项具有挑战性的任务。因此,迫切需要解决能够同时检测多个目标的PEC传感器的开发。
结果:通过水热法成功制备了CdIn2S4/V-MoS2异质结。这些异质结表现出很高的光电流强度,与单独的CdIn2S4相比,增强了1.53倍。接下来,我们设计了一种以ITO为衬底的多通道贴附芯片。通过激光蚀刻产生三个工作电极,然后用CdIn2S4/V-MoS2异质结修饰。然后通过共价键将巯基化的适体自组装到CdIn2S4/V-MoS2异质结上,作为识别工具。以CdIn2S4/V-MoS2异质结为传感平台,以适体为识别工具,我们成功开发了一种一次性aptasensing芯片,用于同时检测三种典型的真菌毒素(黄曲霉毒素B1(AFB1),曲霉毒素A(OTA),和玉米赤霉烯酮(ZEN))。这种aptasensing芯片表现出广泛的AFB1检测范围(0.05-50ng/mL),OTA(0.05-500ng/mL),和ZEN(0.1-250ng/mL)。此外,它显示了AFB1的0.017ng/mL,OTA的0.016ng/mL的超低检测限,和0.033ng/mL的ZEN。
■aptasensing芯片因其成本效益而脱颖而出,制造简单,和多渠道能力。多功能性和实用性使其成为设计多通道PEC传感器的强大平台。凭借其能够以高灵敏度和特异性检测多个目标,aptasensing芯片在不同领域的应用中具有巨大的潜力,如环境监测,临床诊断,和食品安全监管,其中多目标检测至关重要。
BACKGROUND: The importance of multi-target simultaneous detection lies in its ability to significantly boost detection efficiency, making it invaluable for rapid and cost-effective testing. Photoelectrochemical (PEC) sensors have emerged as promising candidates for detecting harmful substances and biomarkers, attributable to their unparalleled sensitivity, minimal background signal, cost-effectiveness, equipment simplicity, and outstanding repeatability. However, designing an effective multi-target detection strategy remains a challenging task in the PEC sensing field. Consequently, there is a pressing need to address the development of PEC sensors capable of simultaneously detecting multiple targets.
RESULTS: CdIn2S4/V-MoS2 heterojunctions were successfully prepared via a hydrothermal method. These heterojunctions exhibited a high photocurrent intensity, representing a 1.53-fold enhancement compared to CdIn2S4 alone. Next, we designed a multi-channel aptasensing chip using ITO as the substrate. Three working electrodes were created via laser etching and subsequently modified with CdIn2S4/V-MoS2 heterojunctions. Thiolated aptamers were then self-assembled onto the CdIn2S4/V-MoS2 heterojunctions via covalent bonds, serving as recognition tool. By empolying the CdIn2S4/V-MoS2 heterojunctions as the sensing platform and aptamers as recognition tool, we successfully developed a disposable aptasensing chip for the simultaneous PEC detection of three typical mycotoxins (aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN)). This aptasensing chip exhibited wide detection range for AFB1 (0.05-50 ng/mL), OTA (0.05-500 ng/mL), and ZEN (0.1-250 ng/mL). Furthermore, it demonstrated ultra-low detection limits of 0.017 ng/mL for AFB1, 0.016 ng/mL for OTA, and 0.033 ng/mL for ZEN.
UNASSIGNED: The aptasensing chip stands out for its cost-effectiveness, simplicity of fabrication, and multi-channel capabilities. The versatility and practicality enable it to serve as a powerful platform for designing multi-channel PEC aptasensors. With its ability to detect multiple targets with high sensitivity and specificity, the aptasensing chip holds immense potential for applications across diverse fields, such as environmental monitoring, clinical diagnostics, and food safety monitoring, where multi-target detection is crucial.