目的:本研究的作者旨在使用微纤维解剖来定义丘脑间粘连(ITA)的显微解剖学,磁共振(MR)纤维束成像,和组织学分析。
方法:矢状,日冕,检查了160名2-82岁健康个体的轴向MR图像。对年龄范围与ITA形态之间以及性别与ITA形态之间的关系进行统计学评估。在这160人中,对100名接受过MR纤维束造影的患者进行了检查。在这个群体中,检查了ITA中纤维束的存在以及与ITA形态类型的关系。还通过内窥镜检查了30个福尔马林固定的人类尸体大脑,在显微镜下从内侧到外侧和上至下方向解剖了6个半球。组织学检查了从具有2型ITA的大脑之一中提取的切片。在组织学检查中使用抗神经丝抗体。
结果:观察到ITA的四种形态类型。1型具有粘附性/粘附性外观,类型2具有桥梁/连缝外观,类型3显示无粘连,和类型4有一个双桥。Tractographic检查显示28%的ITA中没有纤维束过渡,21%有显著的转变,51%的人有一个模糊的转变。统计上,在儿童(年龄)和女性(性别)组中,ITA的发生率明显较高.在具有桥梁/连缝外观的ITA标本中(类型2),纤维束在thalami之间显示出明显的过渡。在类型1(粘附/粘合剂外观)中,在ITA内观察到纤维束,但互惠过渡尚不清楚。解剖显示,ITA中的这些纤维束到达伏隔核,尾状核,前部和前部的额眶区,后部的外侧a和后连合。一些纤维也加入了柄柄。在组织学研究中,用抗神经丝抗体染色观察到在ITA中移动的轴突纤维。
结论:这是第一项通过纤维解剖和透照技术以及放射学和组织学研究证明ITA纤维束的研究。通过比较形态学组与年龄和性别组获得统计数据。这种结构的解剖结构,多年来一直被忽视,被重新检查。这项研究表明,ITA具有连接大脑不同部位的纤维,与以前的研究表明这是一个简单的massa相反。
The authors of this study aimed to define the microanatomy of the interthalamic adhesion (ITA) using microfiber dissection, magnetic resonance (MR) tractography, and histological analysis.
Sagittal, coronal, and axial MR images from 160 healthy individuals 2-82 years of age were examined. The relationships between age range and ITA morphology as well as between gender and ITA morphology were evaluated statistically. Among these 160 individuals, 100 who had undergone MR tractography were examined. In this group, the presence of fiber tracts in the ITA and the relationship with ITA morphological types were examined. Thirty formalin-fixed human cadaveric brains were also examined endoscopically, and 6 hemispheres were dissected from the medial to lateral and superior to inferior directions under the microscope. Sections taken from one of the brains with an ITA type 2 with both thalami were examined histologically. Anti-neurofilament antibody was used in the histological examination.
Four morphological types of ITA were observed. Type 1 had an adhesion/adherent appearance, type 2 had a bridge/commissure appearance, type 3 showed no adhesion, and type 4 had a double bridge. Tractographic examination revealed that 28% had no fiber tract transition in the ITA, 21% had a significant transition, and 51% had an indistinct transition. Statistically, the presence of the ITA was significantly higher in the pediatric (age) and female (gender) groups. In specimens with ITAs of a bridge/commissure appearance (type 2), fiber tracts showed clear transitions between thalami. In type 1 (adherent/adhesive appearance), fiber tracts were observed within the ITA, but a reciprocal transition was unclear. Dissection showed that these fiber tracts in the ITA reach the nucleus accumbens, caudate nucleus, and frontoorbital region anteriorly and the lateral habenula and posterior commissure posteriorly. Some fibers also joined the ansa peduncularis. In histological studies, axonal fibers moving in the ITA were observed with anti-neurofilament antibody staining.
This is the first study to demonstrate fiber tracts of the ITA through fiber dissection and transillumination techniques as well as radiological and histological study. Statistical data were obtained by comparing the morphological group with age and gender groups. The anatomy of this structure, which has been neglected for many years, was reexamined. This study showed that the ITA has fibers connecting different parts of the brain, in contrast to previous studies suggesting that it was a simple massa.