艾美球虫属的球虫是专门的细胞内寄生原生动物,当它们感染宿主时会引起严重的球虫病。感染艾美球虫的动物出现临床症状,如厌食症,腹泻,和便血,甚至会导致死亡.尽管目前治疗球虫病的首选方案是抗生素,这种治疗策略受到抗生素禁令和日益严重的耐药性问题的限制。因此,控制球虫病的替代方法的探索备受关注。植物乳杆菌已被证明具有许多有益作用。在这项研究中,以植物乳杆菌M2为研究对象,通过检测指标,探讨植物乳杆菌对恶性艾美球虫感染小鼠肠道炎症的影响。如卵囊输出,血清细胞因子,和肠道微生物群。与感染组相比,施用植物乳杆菌M2的小鼠的体重减轻百分比显著降低(P<0.05)。补充植物乳杆菌M2和益生菌与双氮唑可显著降低总卵囊产量(P<0.05,P<0.001)。植物乳杆菌M2在维持肠道屏障功能方面表现突出,粘蛋白MUC1和紧密连接蛋白E-cadherin水平显著升高(P<0.01,P<0.05)。研究表明,补充益生菌可缓解感染后的不良反应,显著改善肠道屏障功能。此外,微生态制剂联合双氮齐脲可以优化双氮齐脲的部分功效,这不仅增强了抗生素的作用,而且减轻了它们的副作用。本研究拓展了益生菌的应用,为控制球虫的替代策略提供了新的思路,并为乳酸菌拮抗细胞内病原体感染的相关研究提供了依据。艾美球虫属的IMPORTANCECoccia是专门的细胞内寄生原生动物,目前治疗球虫病的首选方案是抗生素。然而,由于抗生素禁令和耐药性,控制球虫病的替代方法的探索备受关注。在这项工作中,以植物乳杆菌M2为研究对象,发现补充益生菌可以减轻感染后的不良反应,改善肠道屏障功能。这项研究提出了使用乳酸菌控制球虫病的可能性,其潜在机制有待进一步探索。
Coccidia of the genus
Eimeria are specialized intracellular parasitic protozoa that cause severe coccidiosis when they infect their hosts. Animals infected with
Eimeria develop clinical symptoms, such as anorexia, diarrhea, and hematochezia, which can even cause death. Although the current preferred regimen for the treatment of coccidiosis is antibiotics, this treatment strategy is limited by the ban on antibiotics and the growing problem of drug resistance. Therefore, the exploration of alternative methods for controlling coccidiosis has attracted much attention. Lactobacillus plantarum has been shown to have many beneficial effects. In this study, L. plantarum M2 was used as a research object to investigate the effect of L. plantarum on intestinal inflammation induced by infection with
Eimeria falciformis in mice by detecting indicators, such as oocyst output, serum cytokines, and the intestinal microbiota. Compared with that in the infection group, the percent weight loss of the mice that were administered with L. plantarum M2 was significantly reduced (P < 0.05). Supplemented L. plantarum M2 and probiotics combined with diclazuril can reduce the total oocyst output significantly (P < 0.05, P < 0.001). L. plantarum M2 had outstanding performance in maintaining intestinal barrier function, and the levels of the mucin MUC1 and the tight junction protein E-cadherin were significantly elevated (P < 0.01, P < 0.05). Studies have shown that probiotic supplementation can alleviate adverse reactions after infection and significantly improve intestinal barrier function. In addition, probiotics combined with diclazuril could optimize the partial efficacy of diclazuril, which not only enhanced the effect of antibiotics but also alleviated their adverse effects. This study expands the application of probiotics, provides new ideas for alternative strategies for coccidia control, and suggests a basis for related research on lactobacilli antagonizing intracellular pathogen infection.IMPORTANCECoccidia of the genus
Eimeria are specialized intracellular parasitic protozoa, and the current preferred regimen for the treatment of coccidiosis is antibiotics. However, due to antibiotic bans and drug resistance, the exploration of alternative methods for controlling coccidiosis has attracted much attention. In this work, we focused on Lactobacillus plantarum M2 and found that probiotic supplementation can alleviate adverse reactions after infection and improve intestinal barrier function. This study proposes the possibility of using lactic acid bacteria to control coccidiosis, and its potential mechanism needs further exploration.