Abstract：
Eight Eimeria spp. (Apicomplexa: Eimeriidae) have been isolated from the ring-necked pheasant (Phasianus colchicus Linnaeus), native to the temperate zone of Asia and eastern regions of Europe. Enteric coccidiosis has become a major issue associated with the breeding of farmed pheasants for game bird release or meat production. In this study, 35 fecal samples were collected from two-to-three-month-old ring-necked pheasants from four pheasant-rearing farms in Ehime Prefecture, Japan. Microscopic examination using a saturated sugar solution technique detected numerous subspherical oocysts from the samples of one farm and ellipsoidal Eimeria phasiani Tyzzer, 1929 oocysts from the three other farms. The subspherical oocysts were artificially sporulated and measured 18.6 µm by 15.7 µm with a 1.18 shape index (n = 150). Each oocyst contained four 10.7 µm × 5.8 µm sporocysts (n = 30) and one coarse refractile polar granule; no micropyle or residua were detected. Each sporocysts contained two sporozoites with one large and one small refractile body and sparsely distributed residua. The complete, 1,443-bp cytochrome c oxidase subunit I gene (cox1) of this isolate exhibited low sequence identity with published Eimeria spp. sequences including E. phasiani that was previously recorded in the same area. Meanwhile, the oocyst morphology most closely resembled that of Eimeria tetartooimia Wacha, 1973, but with distinct refractile polar granules and sporocyst residua. The available GenBank cox1 sequence of E. tetartooimia exhibited a sequence identity of < 94.5% with the study isolate. Here, the coccidian isolate identified in this study represents a new Eimeria iyoensis n. sp. capable of infecting ring-necked pheasant.
摘要：
八种艾美球虫。（Apicomplex：Eimeriidae）已从环颈野鸡（PhasianuscolchicusLinnaeus）中分离出来，原产于亚洲温带和欧洲东部地区。肠球虫病已成为与养殖野鸡的繁殖有关的主要问题。在这项研究中,从爱媛县四个野鸡饲养农场的两到三个月大的环颈野鸡中收集了35份粪便样本，日本。使用饱和糖溶液技术进行的显微镜检查从一个农场和椭圆形的艾美球虫的样品中检测到许多亚球形卵囊，1929年来自其他三个农场的卵囊。亚球形卵囊被人工孢子化，测量为18.6µmx15.7µm，形状指数为1.18（n=150）。每个卵囊包含四个10.7µm×5.8µm的孢子囊（n=30）和一个粗糙的折射极性颗粒；未检测到微孔或残留物。每个孢子囊包含两个子孢子，一个大的和一个小的折射体和稀疏分布的残余物。完整的,该分离株的1,443-bp细胞色素c氧化酶亚基I基因（cox1）与已发表的艾美球虫属物种具有较低的序列同一性。包括先前记录在同一区域的E.phasiani的序列。同时,卵囊的形态与阿美耳球虫最相似，1973年，但具有明显的折射极性颗粒和孢子囊残留物。可用的E.tartooimia的GenBankcox1序列显示与研究分离物的序列同一性<94.5%。这里,在这项研究中鉴定出的球虫分离株代表了一种新的艾美耳球虫n。sp。能够感染环颈野鸡.
