背景:长期过量饮酒可导致酒精性脂肪肝,构成重大健康风险。茶氨酸(LTA)和表没食子儿茶素没食子酸酯(EGCG)在茶叶中具有抗氧化和保肝作用。然而,LTA和EGCG对酒精性脂肪肝大鼠的联合作用,以及这种影响的潜在机制,仍然不清楚。在这项研究中,SpragueDawley(SD)年夜鼠饲喂酒精6周以引诱酒精性脂肪肝。随后,再过6周,大鼠给予LTA(200mgkg-1day-1),EGCG(200mgkg-1天-1),或LTA与EGCG的组合(40mgkg-1day-1l-Thea+160mgkg-1day-1EGCG),分别。
结果:联合使用LTA和EGCG治疗酒精性脂肪肝的效果比单独给药更显著。这种组合降低了血清天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的活性以及肝脏甘油三酯(TG)的水平,丙二醛(MDA),和大鼠体内的活性氧(ROS)。联合干预还增加了肝超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶的活性。观察到肝脂肪积累和炎症反应的减少。这些作用的机制主要涉及通过下调TNF-α的mRNA和蛋白表达来抑制脂肪酸合成和减轻脂质过氧化,SREBP1c,和CYP2E1以及ADH1,ALDH2,Lipin-1,PPARαPPARα的mRNA和蛋白表达上调,AMPK,和PGC-1α,从而促进脂肪酸的氧化分解,减少胆固醇和葡萄糖的合成。
结论:1-茶氨酸和EGCG似乎能够通过调节脂质代谢和改善氧化应激来减轻酒精性脂肪肝,表明它们作为抗酒精性脂肪肝食品中天然活性成分的潜力。©2024化学工业学会。
BACKGROUND: Chronic excessive alcohol consumption can lead to alcoholic fatty liver, posing substantial health risks. l-Theanine (LTA) and epigallocatechin gallate (
EGCG) in tea exert antioxidant and hepatoprotective effects. However, the combined effects of LTA and
EGCG on rats with alcoholic fatty liver, and the underlying mechanisms of such effects, remain unclear. In this study, Sprague Dawley (SD) rats were fed with alcohol for 6 weeks to induce alcoholic fatty liver. Subsequently, for another 6 weeks, the rats were administered LTA (200 mg kg-1 day-1),
EGCG (200 mg kg-1 day-1), or a combination of LTA with
EGCG (40 mg kg-1 day-1 l-Thea +160 mg kg-1 day-1
EGCG), respectively.
RESULTS: The combined use of LTA and
EGCG for alcoholic fatty liver disease had more significant effects than their individual administration. This combination reduced the activity of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) as well as the levels of hepatic triglyceride (TG), malondialdehyde (MDA), and reactive oxygen species (ROS) in the rats. The combined intervention also increased hepatic superoxide dismutase (SOD) and glutathione peroxidase activity. Reductions in hepatic fat accumulation and inflammatory responses were observed. The mechanism underlying these effects primarily involved the inhibition of fatty acid synthesis and the alleviation of lipid peroxidation through the downregulation of the mRNA and protein expression of TNF-α, SREBP1c, and CYP2E1 and the upregulation of the mRNA and protein expression of ADH1, ALDH2, Lipin-1, PPARαPPARα, AMPK, and PGC-1α, thereby promoting the oxidative decomposition of fatty acids and reducing the synthesis of cholesterol and glucose.
CONCLUSIONS: l-Theanine and EGCG appear to be able to alleviate alcoholic fatty liver by modulating lipid metabolism and ameliorating oxidative stress, indicating their potential as natural active ingredients in anti-alcoholic fatty liver food products. © 2024 Society of Chemical Industry.