背景:隐孢子虫。是世界范围内的原生动物寄生虫,其中包括可导致人类隐孢子虫病的物种。不同的动物物种可以作为疾病传播的宿主和来源,例如啮齿动物物种,因为它们有可能将人畜共患病原体传播给人类和其他动物。在加那利群岛(西班牙),已在腹泻患者中发现了微小隐孢子虫和人隐孢子虫。然而,隐孢子虫的发生。该群岛可能的水库尚不清楚。考虑到这些原生动物的人畜共患潜力,本研究的目的是确定隐孢子虫的存在。在美洲野生啮齿动物中,以及这些哺乳动物作为加那利群岛这些原生动物的传播源的可能作用。
方法:共有来自四个加那利群岛的179只啮齿动物,属于家鼠和家鼠,LaPalma,ElHierro,特内里费岛和兰萨罗特,进行了分析。对粪便中的隐孢子虫进行了筛选。通过18S核糖体RNA片段的巢式PCR和用于系统发育分析的序列。
结果:隐孢子虫。在啮齿动物中广泛分布,总体患病率为12.30%(R.rattus为13.86%,M.m.domesticus为10.25%)。特内里费岛的总体患病率为19.60%,LaPalma的7.14%,ElHierro为5.71%,兰萨罗特为0%。Tyzzeri隐孢子虫,隐孢子虫,隐孢子虫和隐孢子虫。成功鉴定了大鼠基因型I和II/III,除了两种身份不明的隐孢子虫基因型.
结论:这项研究有助于了解隐孢子虫的生物多样性和分布。来自加那利群岛的野生啮齿动物,突出了三种人畜共患物种的存在,C.tyzzeri,C.meleagridis和C.muris,这是在加那利群岛的野生啮齿动物中首次发现这三种物种,也是R.rattus的C.meleagridis的第一份报告。鉴于我们研究中获得的结果,需要在非采样地区进行未来的研究,以更好地了解这些原生动物在群岛野生啮齿动物中的流行病学。
BACKGROUND: Cryptosporidium spp. are worldwide protozoan parasites which include species that can lead to cryptosporidiosis in humans. Different animal species can serve as reservoirs and sources of dissemination of the disease, such as rodent species due their potential in transmitting zoonotic pathogens to humans and other animals. In the Canary Islands (Spain), Cryptosporidium parvum and Cryptosporidium hominis have been identified in patients with diarrhea. However, the occurrence of Cryptosporidium spp. in possible reservoirs in this archipelago remains unclear. Considering the zoonotic potential of these protozoans, the aim of the present study was to determine the presence of Cryptosporidium spp. in peridomestic wild rodents and the possible role of these mammals as a source of transmission of these protozoans in Canary Islands.
METHODS: A total of 179 rodents belonging to Rattus rattus and Mus musculus domesticus from four Canary Islands, La Palma, El Hierro, Tenerife and Lanzarote, were analyzed. Feces were screened for Cryptosporidium spp. by nested PCR of the 18S ribosomal RNA fragment and the sequences used for phylogenetic analyses.
RESULTS: Cryptosporidium spp. were found widely distributed with an overall prevalence of 12.30% in rodents (13.86% for R. rattus and 10.25% for M. m. domesticus). The overall prevalence by island was 19.60% for Tenerife, 7.14% for La Palma, 5.71% for El Hierro and 0% for Lanzarote. Cryptosporidium tyzzeri, Cryptosporidium meleagridis, Cryptosporidium muris and Cryptosporidium sp. rat genotype I and II/III were successfully identified, in addition to two unidentified Cryptosporidium genotypes.
CONCLUSIONS: This study contributes to the knowledge of the biodiversity and distribution of Cryptosporidium spp. in wild rodents from the Canary Islands, highlighting the presence of three zoonotic species, C. tyzzeri, C. meleagridis and C. muris, being the first detection of these three species in wild rodents in the Canary Islands and the first report of C. meleagridis in R. rattus. Given the results obtained in our study, future studies in non-sampled areas are required to better understand the epidemiology of these protozoans in wild rodents in the archipelago.