背景:本研究旨在比较mSEPT9与四种血液标志物(CEA,CA19-9,血小板淋巴细胞比(PLR)和中性粒细胞淋巴细胞比(NLR)。此外,我们的目的是确定mSEPT9,CEA,CA19-9、PLR和NLR在结直肠癌中的表达。
方法:总共567名参与者被纳入研究,包括308例CRC患者,经结肠镜检查和/或组织活检证实的61例结直肠息肉患者和198例健康受试者。收集血浆样品用于测试。
结果:结直肠息肉组mSEPT9阳性率(71.8%)明显高于正常对照组(6.1%)(P<0.001)。CEA的水平,CRC组CA19-9、NLR和PLR明显高于非CRC组(P<0.05)。ROC曲线对比分析显示mSEPT9单独对CRC的诊断效能明显高于CEA,CA19-9,NLR和PLR单独。mSEPT9与CEA的组合,CA19-9和PLR显示出优越的诊断价值。此外,二元logistic回归也用于建立更好的CRC临床诊断模型.在单变量分析中,年龄,mSEPT9,CEA,CA19-9、PLR和NLR是CRC的独立预测因子。当这些协变量在多变量模型中拟合时,mSEPT9、CEA、CA19-9和PLR更可能患有CRC。
结论:这项研究揭示了mSEPT9状态与CRC患者的临床病理特征之间的显著关联,以及mSEPT9,CEA,CA19-9和PLR可显著提高CRC的诊断效能。
BACKGROUND: This study was designed to compare the diagnostic efficacy of mSEPT9 to four blood markers (CEA, CA19-9, platelet-lymphocyte ratio (PLR) and neutrophil-lymphocyte ratio (NLR)). In addition, we aimed to determine the combined diagnostic efficacy of mSEPT9, CEA, CA19-9, PLR and NLR in colorectal cancer.
METHODS: A total of 567 participants were enrolled in the study, including 308 CRC patients, 61 colorectal polyp patients and 198 healthy subjects confirmed by colonoscopy and/or tissue biopsy. Plasma samples were collected for tests.
RESULTS: The positive rate of mSEPT9 in CRC (71.8%) was markedly higher than that in either the colorectal polyps group (27.9%) or the healthy controls (6.1%) (P < 0.001). The levels of CEA, CA19-9, NLR and PLR in the CRC group were significantly higher than those in the non-CRC groups (P < 0.05). ROC curves comparison analyses showed that the diagnostic efficacy of mSEPT9 alone in CRC was significantly higher than CEA, CA19-9, NLR and PLR alone. The combination of mSEPT9 with CEA, CA19-9 and PLR showed superior diagnostic value. In addition, binary logistic regression was also used to build a better model for clinical diagnosis of CRC. On univariable analyses, age, mSEPT9, CEA, CA 19-9, PLR and NLR were independent predictors of CRC. When these covariates were fitted in multivariable models, the ones with positive detection of mSEPT9, CEA, CA 19-9 and PLR were more likely to have CRC.
CONCLUSIONS: This research revealed a significant association between mSEPT9 status and the clinicopathological characteristics of CRC patients, and the combination of mSEPT9, CEA, CA19-9 and PLR could significantly improve diagnostic efficacy in CRC.