Abstract：
Biomarkers screening is a benefit approach for early diagnosis of major diseases. In this study, magnetic nanoparticles (MNPs) have been utilized as labels to establish a multi-line immunochromatography (MNP-MLIC) for simultaneous detection of carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA 19-9), and alpha-fetoprotein (AFP) in a single serum sample. Under the optimal parameters, the three biomarkers can be rapidly and simultaneously qualitative screening within 15 min by naked eye. As for quantitative detection, the MNP-MLIC test strips were precisely positioned and captured by a smartphone, and signals on the test and control lines were extracted by ImageJ software. The signal ratio of test and control lines has been calculated and used to plot quantitative standard curves with the logarithmic concentration, of which the correlation coefficients are more than 0.99, and the limit of detection for CEA, CA 19-9, and AFP were 0.60 ng/mL, 1.21 U/mL, and 0.93 ng/mL, respectively. The recoveries of blank serum were 75.0 ~ 112.5% with the relative standard deviation ranging from 2.5 to 15.3%, and the specificity investigation demonstrated that the MNP-MLIC is highly specific to the three biomarkers. In conclusion, the developed MNP-MLIC offers a rapid, simple, accurate, and highly specific method for simultaneously detecting multiple biomarkers in serum samples, which provides an efficient and accurate approach for the early diagnosis of diseases.
摘要：
生物标志物筛选是重大疾病早期诊断的有益方法。在这项研究中,磁性纳米颗粒（MNPs）已被用作标记，以建立同时检测癌胚抗原（CEA）的多线免疫层析（MNP-MLIC），碳水化合物抗原199（CA19-9），和甲胎蛋白（AFP）在一个单一的血清样品。在最优参数下，3种生物标志物可以在15min内通过肉眼快速同时定性筛选。至于定量检测，MNP-MLIC测试条被智能手机精确定位和捕获，通过ImageJ软件提取测试和控制线上的信号。已计算出测试线和对照线的信号比，并用于绘制对数浓度的定量标准曲线，其中相关系数大于0.99，CEA的检测限，CA19-9，AFP为0.60ng/mL，1.21U/mL,和0.93ng/mL，分别。空白血清的加标回收率为75.0~112.5%,相对标准偏差为2.5~15.3%,特异性研究表明，MNP-MLIC对这三种生物标志物具有高度特异性。总之,开发的MNP-MLIC提供了一种快速的，简单,准确,同时检测血清样品中多种生物标志物的高特异性方法，为疾病的早期诊断提供了一种高效、准确的方法。
