PDF(Pubmed)
Abstract：
Specific markers for colorectal cancer (CRC), preceded by colorectal adenoma (pre-CRC), are lacking. This study aimed to investigate whether microRNAs (miR-19a-3p, miR-92a-3p, miR-193a-3p, and miR-210-3p) from tissues and exosomes are potential CRC biomarkers and compare them to existing biomarkers, namely carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9. MiRNA was isolated in the samples of 52 CRC and 76 pre-CRC patients. Expression levels were analyzed by RT-qPCR. When comparing pre-CRC and CRC tissue expression levels, only miR-193a-3p showed statistically significant result (p < 0.0001). When comparing the tissues and exosomes of CRC samples, a statistically significant difference was found for miR-193a-3p (p < 0.0001), miR-19a-3p (p < 0.0001), miR-92a-3p (p = 0.0212), and miR-210-3p (p < 0.0001). A receiver-operating characteristic (ROC) curve and area under the ROC curve (AUC) were used to evaluate the diagnostic value of CEA, CA 19-9, and miRNAs. CEA and CA 19-9 had good diagnostic values (AUCs of 0.798 and 0.668). The diagnostic value only of miR-193a-3p was highlighted (AUC = 0.725). The final logistic regression model, in which we put a combination of CEA concentration and the miR-193a-3p expression level in tissues, showed that using these two markers can distinguish CRC and pre-CRC in 71.3% of cases (AUC = 0.823). MiR-193a-3p from tissues could be a potential CRC biomarker.
摘要：
结直肠癌（CRC）的特异性标志物，之前是结直肠腺瘤（CRC前），缺乏。本研究旨在探讨microRNAs(miR-19a-3p,miR-92a-3p,miR-193a-3p,来自组织和外泌体的miR-210-3p）是潜在的CRC生物标志物，并将它们与现有的生物标志物进行比较，即癌胚抗原(CEA)和糖类抗原(CA)19-9。在52例CRC和76例CRC前患者的样品中分离miRNA。通过RT-qPCR分析表达水平。当比较pre-CRC和CRC组织表达水平时，只有miR-193a-3p显示出统计学上显著的结果(p<0.0001)。当比较CRC样本的组织和外泌体时，发现miR-193a-3p有统计学意义的差异（p<0.0001），miR-19a-3p（p<0.0001），miR-92a-3p（p=0.0212），和miR-210-3p(p<0.0001)。采用受试者工作特征(ROC)曲线和ROC曲线下面积(AUC)评价CEA的诊断价值,CA19-9和miRNA。CEA和CA19-9具有良好的诊断价值（AUC为0.798和0.668）。仅miR-193a-3p的诊断价值被强调(AUC=0.725)。最终的逻辑回归模型，其中我们将CEA浓度和组织中miR-193a-3p表达水平相结合，显示使用这两种标志物可以在71.3%的病例中区分CRC和CRC前(AUC=0.823)。来自组织的MiR-193a-3p可能是潜在的CRC生物标志物。
