毛状体是位于植物表皮中的专门器官,对生物和非生物胁迫起着重要的防御作用。然而,调控辣椒表皮毛状体发育的分子机制及相关调控基因尚不清楚。因此,我们对A114(较少毛状体)和A115(较多毛状体)进行了转录组分析,以深入研究辣椒表皮毛状体发育调控机制中涉及的基因.在这项研究中,通过表型观察发现A115的表皮毛状体密度较高,并且在开花期叶片中最高。通过RNA-Seq对总共39,261个基因进行了定量,包括先前基因组分析中未注释的11,939个基因和18,833个差异表达基因。基于KEGG功能富集,发现DEGs主要集中在三个途径:植物-病原体相互作用,MAPK信号通路-植物,和植物激素信号转导。我们进一步筛选了与辣椒表皮毛状体发育相关的DEGs,以及植物信号基因GID1B样(Capana03g003488)和PR-6(Capana09g001847)的表达,转录因子MYB108(Capana05g002225)和ABR1样(Capana04g001261),DEGs中的植物抗性基因PGIP-like(Capana09g002077)和At5g49770(Capana08g001721)在A115高于A114,并且在开花期在叶片中高度表达。此外,基于WGCNA结果和共表达网络的建立表明,上述基因之间呈高度正相关。本研究的转录组学数据和分析为研究辣椒表皮毛状体的调控机制提供了基础。
Trichomes are specialized organs located in the plant epidermis that play important defense roles against biotic and abiotic stresses. However, the mechanisms regulating the development of pepper epidermal trichomes and the related regulatory genes at the molecular level are not clear. Therefore, we performed transcriptome analyses of A114 (less
trichome) and A115 (more
trichome) to dig deeper into the genes involved in the regulatory mechanisms of epidermal
trichome development in peppers. In this study, the epidermal
trichome density of A115 was found to be higher by phenotypic observation and was highest in the leaves at the flowering stage. A total of 39,261 genes were quantified by RNA-Seq, including 11,939 genes not annotated in the previous genome analysis and 18,833 differentially expressed genes. Based on KEGG functional enrichment, it was found that DEGs were mainly concentrated in three pathways: plant-pathogen interaction, MAPK signaling pathway-plant, and plant hormone signal transduction. We further screened the DEGs associated with the development of epidermal trichomes in peppers, and the expression of the plant signaling genes GID1B-like (Capana03g003488) and PR-6 (Capana09g001847), the transcription factors MYB108 (Capana05g002225) and ABR1-like (Capana04g001261), and the plant resistance genes PGIP-like (Capana09g002077) and At5g49770 (Capana08g001721) in the DEGs were higher at A115 compared to A114, and were highly expressed in leaves at the flowering stage. In addition, based on the WGCNA results and the establishment of co-expression networks showed that the above genes were highly positively correlated with each other. The transcriptomic data and analysis of this study provide a basis for the study of the regulatory mechanisms of pepper epidermal trichomes.