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Abstract：
Cotton is one of the most important natural fibers used in the textile industry worldwide. It is important to identify the key factors involved in cotton fiber development. In this study, zinc finger protein8 (GhZFP8) encoding a C2H2 transcription factor (TF) was cloned from cotton. qPCR showed that the transcripts of GhZFP8 in cotton were detected in the leaves and fibers at 3, 6, and 30 days post-anthesis (DPA), but not in the roots, stems, or flowers. The overexpression of GhZFP8 increased the trichome number on the siliques, leaves, and inflorescence, but inhibited the growth. The expression of trichome development and cell-elongation-related genes decreased obviously in GhZFP8 overexpressor Arabidopsis. Indole-3-acetic acid (IAA) and 1-Aminocyclopropanecarboxylic acid (ACC) contents were much higher in GhZFP8 overexpressors than that found in the wild type, but the gibberellin (GA) content was lower. The interference of GhZFP8 in cotton caused smaller bolls and shorter fibers than that of the control. The results of DNA affinity purification (DAP)-seq showed that GhZFP8 could bind to the promoter, exon, intron, and intergenic region of the target genes, which are involved in photosynthesis, signal transduction, synthesis of biomass, etc. Our findings implied that GhZFP8 processed multiple biological functions and regulated the development of cotton fiber.
摘要：
棉花是世界范围内纺织工业中最重要的天然纤维之一。重要的是找出影响棉纤维发育的症结身分。在这项研究中,从棉花中克隆了编码C2H2转录因子（TF）的锌指蛋白8（GhZFP8）。qPCR表明，在花后3、6和30天（DPA）的叶片和纤维中检测到棉花中GhZFP8的转录本，但不是在根部，茎,或鲜花。GhZFP8的过表达增加了长角果上的毛状体数量，叶子,和花序，但抑制了增长。在GhZFP8过表达拟南芥中，毛状体发育和细胞伸长相关基因的表达明显下降。GhZFP8过表达物中吲哚-3-乙酸（IAA）和1-氨基环丙烷甲酸（ACC）的含量远高于野生型，但赤霉素(GA)含量较低。GhZFP8在棉花中的干扰导致比对照更小的棉铃和更短的纤维。DNA亲和纯化(DAP)-seq结果表明GhZFP8能与启动子结合,外显子,内含子,和靶基因的基因间区域，参与光合作用，信号转导，生物质的合成，等。我们的发现表明GhZFP8处理了多种生物学功能并调节了棉纤维的发育。
