PDF(Pubmed)
Abstract：
Melon pest management relies on the excessive application of pesticides. Reducing pesticide spraying has become a global issue for environmental sustainability and human health. Therefore, developing a new cropping system that is sustainable and eco-friendly is important. This study found that melon seedlings irrigated with ultrafine water containing H2 and O2 (UFW) produced more root hairs, increased shoot height, and produced more flowers than the control irrigated with reverse osmosis (RO) water. Surprisingly, we also discovered that UFW irrigation significantly reduced aphid infestation in melons. Based on cryo-scanning electron microscope (cryo-SEM) observations, UFW treatment enhanced trichome development and prevented aphid infestation. To investigate whether it was H2 or O2 that helped to deter insect infestation, we prepared UF water enrichment of H2 (UF+H2) and O2 (UF+O2) separately and irrigated melons. Cryo-SEM results indicated that both UF+H2 and UF+O2 can increase the density of trichomes in melon leaves and petioles. RT-qPCR showed that UF+H2 significantly increased the gene expression level of the trichome-related gene GLABRA2 (GL2). We planted melons in a plastic greenhouse and irrigated them with ultrafine water enrichment of hydrogen (UF+H2) and oxygen (UF+O2). The SPAD value, photosynthetic parameters, root weight, fruit weight, and fruit sweetness were all better than the control without ultrafine water irrigation. UFW significantly increased trichome development, enhanced insect resistance, and improved fruit traits. This system thus provides useful water management for pest control and sustainable agricultural production.
摘要：
甜瓜害虫的管理依赖于农药的过度施用。减少农药喷洒已成为环境可持续性和人类健康的全球性问题。因此,发展可持续和生态友好的新种植系统非常重要。这项研究发现，用含有H2和O2的超细水（UFW）灌溉的甜瓜幼苗产生更多的根毛，增加射击高度，并且比用反渗透（RO）水灌溉的对照产生更多的花。令人惊讶的是,我们还发现UFW灌溉可显着减少甜瓜中的蚜虫侵染。基于低温扫描电子显微镜(cryo-SEM)观察,UFW治疗可增强毛状体的发育并防止蚜虫侵染。为了调查是H2还是O2有助于阻止昆虫侵扰，我们分别制备了H2（UFH2）和O2（UFO2）的UF水富集和灌溉甜瓜。Cryo-SEM结果表明，UFH2和UFO2均可增加甜瓜叶片和叶柄中毛状体的密度。RT-qPCR显示UF+H2显著提高了毛状体相关基因GLABRA2(GL2)的基因表达水平。我们在塑料温室中种植了甜瓜，并用富含氢（UFH2）和氧（UFO2）的超细水灌溉。SPAD值,光合参数，根重,水果重量,果实甜度均优于未进行超细水灌溉的对照。UFW显著增加了毛状体的发育，增强抗虫性，和改善果实性状。因此，该系统为病虫害控制和可持续农业生产提供了有用的水管理。
