诱变是建立遗传变异的高效工具,广泛用于各种植物的遗传增强。突变育种的关键好处是在不改变遗传背景的情况下增强品种的一个或几个特征的前景。在这项研究中,我们将丹参的种子暴露于四种浓度的水合肼(HZ)中,即,(0%,0.1%,0.2%,和0.3%)持续6小时。通过GC-MS测定了由HZ处理的种子驱动的S.officinalis幼苗中萜类化合物的含量,共鉴定出340种植物化学物质;163种(87.48%),145(84.49%),65(97.45%),和62(98.32%),从四个浓度的HZ(0%,0.1%,0.2%,和0.3%),分别。此外,我们使用qRT-PCR系统揭示了与萜类和萜烯生物合成相关的12个TPS基因的“转录控制”,即,SoGPS,SoMYRS,SoNEOD,SoCINS,SoSABS,索林斯,SoFPPS,SoHUMS,SoTPS6,SoSQUS,SoGGPS,SoGA2总之,结果可能会确保用于处理种子的化学诱变剂HZ的浓度之间存在一定的正相关关系,生产的萜烯的类型和数量,以及它们相应基因的表达。
Mutagenesis is a highly efficient tool for establishing genetic variation and is widely used for genetic enhancement in various plants. The key benefit of mutation breeding is the prospect of enhancing one or several characteristics of a variety without altering the genetic background. In this study, we exposed the seeds of Salvia officinalis to four concentrations of hydrazine hydrate (HZ), i.e., (0%, 0.1%, 0.2%, and 0.3%) for 6 h. The contents of terpenoid compounds in the S. officinalis plantlets driven from the HZ-treated seeds were determined by GC-MS, which resulted in the identification of a total of 340 phytochemical compounds; 163 (87.48%), 145 (84.49%), 65 (97.45%), and 62 (98.32%), from the four concentrations of HZ (0%, 0.1%, 0.2%, and 0.3%), respectively. Furthermore, we used the qRT-PCR system to disclose the \"transcriptional control\" for twelve TPS genes related to terpenoid and terpene biosynthesis, namely, SoGPS, SoMYRS, SoNEOD, SoCINS, SoSABS, SoLINS, SoFPPS, SoHUMS, SoTPS6, SoSQUS, SoGGPS, and SoGA2. Altogether, results are likely to ensure some positive relationship between the concentrations of the chemical mutagen HZ used for treating the seeds, the type and amount of the produced terpenes, and the expression of their corresponding genes.