人工蚕食显著影响农场盈利能力。可持续的蚕茧生产取决于饲料效率的不断提高,以降低成本和饲料中的营养损失。本研究采用代谢组学方法探讨人工饮食和桑叶饮食两种饲养方式下蚕茧和血淋巴的差异。桑叶组蚕茧和血淋巴的9种代谢产物高于人工饮食组。这些代谢物的KEGG途径的富集分析表明,它们主要富集在缬氨酸中,亮氨酸,和异亮氨酸的生物合成和降解途径。因此,人工家蚕日粮补充了各种浓度的缬氨酸,目的是研究缬氨酸对人工日粮的摄食和消化的影响。结果表明,缬氨酸的添加对五龄家蚕的饲料消化率没有显著影响。2%和4%缬氨酸组的食物摄取显著低于0%缬氨酸组。然而,2%和4%缬氨酸组显着提高了蚕茧生产效率,分别上涨11.3%和25.1%,分别。然而,2%和4%缬氨酸组的茧层生产效率分别下降了7.7%和13.9%,分别。研究证实缬氨酸是提高家蚕饲料效率的有效物质。
Artificial
silkworm diets significantly impact farm profitability. Sustainable cocoon production depends on the continuous improvement of feed efficiency to reduce costs and nutrient losses in the feed. This study used metabolomics to explore the differences in
silkworm cocoons and hemolymph under two modes of rearing: an artificial diet and a mulberry-leaf diet. Nine metabolites of
silkworm cocoons and hemolymph in the mulberry-leaf group were higher than those in the artificial-diet group. Enrichment analysis of the KEGG pathways for these metabolites revealed that they were mainly enriched in the valine, leucine, and isoleucine biosynthesis and degradation pathways. Hence, the artificial
silkworm diet was supplemented various concentrations of valine were supplemented to with the aim of examining the impact of valine on their feeding and digestion of the artificial diet. The results indicated that valine addition had no significant effect on feed digestibility in the fifth-instar
silkworm. Food intake in the 2% and 4% valine groups was significantly lower than that in the 0% valine group. However, the 2% and 4% valine groups showed significantly improved cocoon-production efficiency, at 11.3% and 25.1% higher, respectively. However, the cocoon-layer-production efficiencies of the 2% and 4% valine groups decreased by 7.7% and 13.9%, respectively. The research confirmed that valine is an effective substance for enhancing the feed efficiency of silkworms.