家蚕核型多角体病毒(BmNPV)是威胁蚕桑产业的最重要病毒。目前,对于家蚕的BmNPV感染没有有效的治疗方法,lncRNA在生物免疫应答和宿主病毒相互作用中起着重要作用,但是对蚕的研究相对较少。在这项研究中,抗性菌株NB(NB)和易感菌株306(306)的四个中肠组织样本以及连续感染BmNPV96小时的NB和306进行全转录组测序,分析NB和306遗传背景的差异以及接种BmNPV后的差异,和显著不同的mRNA,筛选BmNPV接种后NB和306之间的miRNA和lnRNA。通过比较NB和306,2,651个显著不同的mRNA,筛选了57个显著不同的miRNAs和198个显著不同的lncRNAs。通过比较接种BmNPV后的NB和306,2684个显著不同的mRNA,筛选了39个显著不同的miRNA和125个显著不同的lncRNA。根据mRNA的显著差异,病毒接种后从NB和306以及NB和306中筛选的miRNA和lncRNA,在病毒接种前后构建mRNA-miRNA-lncRNA调控网络,并从中筛选出BmBCAT-Bomo_chr7_8305-MSTRG.3236.2调节轴,发现BmBCAT在遗传背景下不受Bomo_chr7_8305调节,病毒感染后,MSTRG.3236.2竞争结合Bomo_chr7_8305调节BmBCAT。通过qPCR验证全转录组测序结果,并进行时间序列表达分析以证明调控网络的可靠性。BmBCAT-Bomo_chr7_8305-MSTRG.3236.2调节轴可能在蚕与BmNPV的相互作用中起潜在作用。这些结果为家蚕与BmNPV之间的相互作用机制提供了新的见解。
Bombyx mori nucleopolyhedrovirus (BmNPV) is the most important virus that threatens sericulture industry. At present, there is no effective treatment for BmNPV infection in silkworms, and lncRNA plays an important role in biological immune response and host-virus interaction, but there are relatively few studies in silkworms. In this
study, the four midgut tissue samples of the resistance strain NB (NB) and susceptible strain 306 (306) and the NB and 306 continuously infected with BmNPV for 96 h are used for whole transcriptome sequencing to analyze the differences in the genetic background of NB and 306 and the differences after inoculation of BmNPV, and the significantly different mRNA, miRNA and lnRNA between NB and 306 after BmNPV inoculation were screened. By comparing NB and 306, 2651 significantly different mRNAs, 57 significantly different miRNAs and 198 significantly different lncRNAs were screened. By comparing NB and 306 after BmNPV inoculation, 2684 significantly different mRNAs, 39 significantly different miRNAs and 125 significantly different lncRNAs were screened. According to the significantly different mRNA, miRNA and lncRNA screened from NB and 306 and NB and 306 after virus inoculation, the mRNA-miRNA-lncRNA regulatory network was constructed before and after virus inoculation, and the BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 regulatory axis was screened from them, and it was found that BmBCAT was not Bomo_chr7_8305 regulated in the genetic background, after viral infection, MSTRG.3236.2 competes for binding Bomo_chr7_8305 regulates BmBCAT. The whole transcriptome sequencing results were verified by qPCR and the time-series expression analysis was performed to prove the reliability of the regulatory network. The BmBCAT-Bomo_chr7_8305-MSTRG.3236.2 regulatory axis may play a potential role in the interaction between silkworms and BmNPV. These results provide new insights into the interaction mechanism between silkworms and BmNPV.