苦荞麦(Fagopyrumtataricum)富含多糖,可被肠道微生物群(GM)利用,并提供多种健康益处。然而,这些多糖的作用机制至今仍不清楚.在这项研究中,苦荞麦多糖(TBP)的纯化,并获得五个级分。使用离子色谱法测定这些级分的组成。研究了不同TBP成分对三种双歧杆菌和鼠李糖乳杆菌的益生菌作用。此外,评估了TBP对GM和短链脂肪酸(SCFAs)的影响。结果表明,TBP部分的益生菌作用取决于其组成。存在于不同组分中的多糖具有特定的益生菌作用。TBP-1.0,主要由岩藻糖组成,葡萄糖,和d-半乳糖,对鼠李糖乳杆菌表现出最强的增殖作用,而TBP-W,富含葡萄糖,D-半乳糖,和果糖,对长双歧杆菌和青春双歧杆菌的生长促进作用最好。此外,TBP-0.2,由D-半乳糖醛酸组成,D-半乳糖,木糖,和阿拉伯糖,对短双歧杆菌生长的影响最大。在粪便发酵过程中添加TBP会显著改变GM的组成,随着乳球菌相对丰度的增加,相颈杆菌,拟杆菌,和志贺氏菌.同时,TBP也显著提高了SCFA水平.我们的发现表明,苦荞麦可以提供特定的膳食多糖来源来调节和维持转基因多样性。它们为苦荞麦商业化利用转基因调制提供了基础。
Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits. However, the mechanisms underlying the action of these polysaccharides remain unclear to date. In this study, Tartary buckwheat polysaccharides (TBP) were purified, and five fractions were obtained. The composition of these fractions was determined using ion chromatography. Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus. In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated. Results showed that the probiotic effect of TBP fraction was dependent on their composition. The polysaccharides present in different fractions had specific probiotic effects. TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth. Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth. The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella. Simultaneously, the level of SCFA was also significantly increased by TBP. Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity. They provide a basis for Tartary buckwheat commercial utilization for GM modulation.