PDF(Pubmed)
Abstract：
The human gastrointestinal (GI) tract harbors diverse microbes, and the family Lachnospiraceae is one of the most abundant and widely occurring bacterial groups in the human GI tract. Beneficial and adverse effects of the Lachnospiraceae on host health were reported, but the diversities at species/strain levels as well as their metabolites of Lachnospiraceae have been, so far, not well documented. In the present study, we report on the collection of 77 human-originated Lachnospiraceae species (please refer hLchsp, https://hgmb.nmdc.cn/subject/lachnospiraceae) and the in vitro metabolite profiles of 110 Lachnospiraceae strains (https://hgmb.nmdc.cn/subject/lachnospiraceae/metabolites). The Lachnospiraceae strains in hLchsp produced 242 metabolites of 17 categories. The larger categories were alcohols (89), ketones (35), pyrazines (29), short (C2-C5), and long (C > 5) chain acids (31), phenols (14), aldehydes (14), and other 30 compounds. Among them, 22 metabolites were aromatic compounds. The well-known beneficial gut microbial metabolite, butyric acid, was generally produced by many Lachnospiraceae strains, and Agathobacter rectalis strain Lach-101 and Coprococcus comes strain NSJ-173 were the top 2 butyric acid producers, as 331.5 and 310.9 mg/L of butyric acids were produced in vitro, respectively. Further analysis of the publicly available cohort-based volatile-metabolomic data sets of human feces revealed that over 30% of the prevailing volatile metabolites were covered by Lachnospiraceae metabolites identified in this study. This study provides Lachnospiraceae strain resources together with their metabolic profiles for future studies on host-microbe interactions and developments of novel probiotics or biotherapies.
摘要：
人类胃肠道（GI）藏有多种微生物，Lachnospirosaceae家族是人类胃肠道中最丰富和广泛存在的细菌群之一。据报道，Lachnospirosaceae对宿主健康的有益和不利影响，但是Lachnospiraceae的物种/菌株水平及其代谢物的多样性，到目前为止,没有很好的记录。在本研究中,我们报告了77种人类起源的落叶松科物种的收集(请参阅hLchsp，https://hgmb.nmdc.cn/subject/lachnosispileae）和110个laachnosispileae菌株的体外代谢谱（https://hgmb。nmdc.cn/受试者/幼虫科/代谢物)。hLchsp中的落叶松科菌株产生了17类的242种代谢产物。较大的类别是酒精（89），酮（35），吡嗪类(29),短路（C2-C5），和长(C>5)链酸(31)，酚类(14)，醛(14)，和其他30种化合物。其中,22种代谢物为芳香族化合物。众所周知的有益肠道微生物代谢产物，丁酸,通常由许多落叶草科菌株产生，和直肠不动杆菌菌株Lach-101和coprococcuscomes菌株NSJ-173是前2个丁酸生产者，当体外产生331.5和310.9mg/L的丁酸时，分别。对公开的基于队列的人类粪便挥发性代谢组学数据集的进一步分析显示，超过30％的主要挥发性代谢产物被本研究中确定的Lachnospiraceae代谢产物所覆盖。这项研究提供了Lachnoshispileaceae菌株资源及其代谢谱，用于未来研究宿主-微生物相互作用以及新型益生菌或生物疗法的开发。
