在格陵兰和斯瓦尔巴群岛进行了采样活动,以探索寒冷栖息地的真菌多样性。发现了三组非常丰富的菌株,由最近描述的或尚未描述的嗜冷和寡营养酵母和双态真菌组成,在我们的研究中,约占担子菌可培养总多样性的50%。这些分类单元的出现也已通过与培养无关的方法得到证明。基于核糖体基因簇序列的系统发育分析(28S(LSU)的D1/D2结构域,18S(SSU),具有5.8SrDNA的ITS)和延伸因子一α(TEF)的蛋白质编码基因序列,细胞色素b(CYTB)和从纯培养物中获得的RNA聚合酶II的两个亚基(RPB1和RPB2),本研究中提出的分离类群属于担子菌,puciniomcotina亚门,类微生物,植物科。序列的数据集支持对三种物种的识别:Camptobasilumgelus,大竹。11月。(前型菌株EXF-12713)和冰心菌。和sp。11月。(外型菌株EXF-13111)。在斯瓦尔巴群岛和格陵兰的样本中发现了甘草凝胶,而这里提出的新物种的代表,C.北极,只在格陵兰冰盖中发现.嗜肉菌。11月。为在斯瓦尔巴群岛和格陵兰冰川环境中发现的双态/丝状分离物竖立。出租车,对其使用了无效的名称\'Rhodotorulasvalbardensis\',属于这个属。基于核糖体基因,弓形虫和冰心菌是相关的,系统发育上与冰川酶属和冰毒属最密切相关。七个基因的系统发育仅限于具有可用序列的分类群,支持将嗜冰菌放置在Camptobasiaceae中。
Sampling campaigns in Greenland and Svalbard were executed to explore fungal diversity in cold habitats. Three very abundant groups of strains were discovered, consisting either of recently described or of yet-undescribed psychrophilic and oligotrophic yeasts and dimorphic fungi, accounting for around 50 % of the total cultivable diversity of basidiomycetes in our studies. The occurrence of these taxa has also been demonstrated by culture-independent methods. Based on phylogenetic analyses of ribosomal gene cluster sequences (D1/D2 domains of 28S (LSU), 18S (SSU), ITS with 5.8S rDNA) and sequences of protein-coding genes for elongation factor one alpha (TEF), cytochrome b (CYTB) and two subunits of the RNA polymerase II (RPB1 and RPB2) obtained from pure cultures, the isolated taxa presented in this study belong to Basidiomycota, subphylum Pucciniomycotina, class Microbotryomycetes, family Camptobasidiaceae. The dataset of the sequences supported the recognition of three species: Camptobasidium gelus, Camptobasidium arcticum sp. nov. (ex-type strain EXF-12713) and Psychromyces glacialis gen. and sp. nov. (ex-type strain EXF-13111). Camptobasidium gelus was found in the Svalbard and Greenland samples, while representatives of the here proposed new species, C. arcticum, were found only in the Greenland Ice Sheet. Psychromyces gen. nov. was erected for the dimorphic/
filamentous isolates found in Svalbard and Greenland glacial environments. The taxon, for which the invalid name \'Rhodotorula svalbardensis\' has been used, belongs to this genus. Based on ribosomal genes, Camptobasidium arcticum and Psychromyces glacialis are related, phylogenetically most closely related to the genera Glaciozyma and Cryolevonia. Seven genes phylogeny restricted to taxa with available sequences, supported the placement of Psychromyces to Camptobasidiaceae.