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Abstract：
Biofilm-forming cyanobacteria are abundant in mangrove ecosystems, colonizing various niches including sediment surface and periphyton where they can cover large areas, yet have received limited attention. Several filamentous isolates were recently isolated from Guadeloupe, illustrating the diversity and novelty present in these biofilms. In this study, nine strains belonging to three novel lineages found abundantly in Guadeloupe biofilms are characterized by genome sequencing, morphological and ultrastructural examination, metabolome fingerprinting and searched for secondary metabolites biosynthesis pathways. Assignation of two lineages to known genera is confirmed, namely Scytonema and Jaaginema. The third lineage corresponds to a new Coleofasciculales genus herein described as Karukerafilum gen. nov. The four strains belonging to this genus group into two subclades, one of which displays genes necessary for nitrogen fixation as well as the complete pathway for geosmin production. This study gives new insights into the diversity of mangrove biofilm-forming cyanobacteria, including genome-based description of a new genus and the first genome sequence available for the genus Jaaginema.
摘要：
生物膜形成蓝细菌在红树林生态系统中含量丰富，殖民各种生态位，包括沉积物表面和附着物，它们可以覆盖大面积，但受到的关注有限。最近从瓜德罗普岛分离出几种丝状分离物，说明了这些生物膜中存在的多样性和新颖性。在这项研究中,在瓜德罗普岛生物膜中大量发现的属于三个新谱系的9个菌株的特征在于基因组测序，形态学和超微结构检查，代谢组指纹分析并搜索次级代谢物生物合成途径。确认将两个谱系分配给已知属，即Scytonema和Jaaginema。第三谱系对应于本文描述为Karukerafilumgen的新Coleofascicula属。11月。将属于本属的四个菌株分为两个亚进化枝，其中之一显示了固氮所必需的基因以及产生地蛋白的完整途径。这项研究为红树林生物膜形成蓝细菌的多样性提供了新的见解，包括对新属的基于基因组的描述和可用于Jaaginema属的第一个基因组序列。
