The management of nitrogen (N) fertilization is of fundamental importance in hydroponics. To reduce the supply of nitrate (NO3 -) in fertigation recipes for Batavia lettuce crops grown in closed hydroponics, partial replacement of nitrate by chloride (NO3 -/Cl-) at different ratios but with the same equivalent sum was experimentally tested. The experiment included four nutritional treatments in the replenishment nutrient solution, particularly T1; 0.7 mM Cl-/19 mM NO3 -, T2; 2 mM Cl-/17.7 mM NO3 -, T3; 4 mM Cl-/15.7 mM NO3 - and T4; 6 mM Cl-/13.7 mM NO3 -. The results showed that reducing nitrate supply combined with equivalent increase in chloride application gradually reduced the gap between nitrate input and nitrogen uptake concentrations, with the smallest differences occurring in T4 treatment, which reduced the nitrate concentration in the drainage by 50%. The tested treatments led to very small variations in plant water uptake, production of fresh biomass and nutritional quality, which is justified by the proper functioning of key physiological mechanisms, such as stomatal conductance, which was followed by an increased efficiency of nitrogen use up to 25% (kg fresh biomass kg-1 N supply). The steady level of C/N ratio in the plant tissue irrespective of NO3 -/Cl- supply ratio points to sufficiency in photosynthetic products and adequacy in the supply of nitrogen, although leaf Cl- content increased up to 19.6 mg g-1 dry weight in the lowest NO3 -/Cl- treatment. Nutrient uptake concentrations were determined as follows: 13.4 (N), 1.72 (P), 10.2 (K), 3.13 (Ca), 0.86 (Mg, mmol L-1), 27.8 (Fe), 5.63 (Mn), 5.45 (Zn) and 0.72 (Cu, μmol L-1). This study suggests that replacing 30% of NO3 - supply with Cl- in fertigation recipes for hydroponic lettuce crops reduces leaf nitrate content without affecting physiological processes, growth, and quality, verifying in parallel the role of chloride as a beneficial macronutrient. Finally, a relationship between Cl- uptake and its concentration in the root zone solution was established enabling the simulation of chloride to water consumption.

All terrestrial vertebrate life must transition from aquatic gas exchange in the embryonic environment to aerial or pulmonary respiration at birth. In addition to being able to breathe air, neonates must possess functional sensory feedback systems for maintaining acid-base balance. Respiratory neurons in the brainstem act as pH sensors that can adjust breathing to regulate systemic pH. The central pH sensitivity of breathing-related motor output develops over the embryonic period in the zebra finch (Taeniopygia guttata). Due to the key role of chloride ions in electrochemical stability and developmental plasticity, we tested chloride\'s role in the development of central pH sensitivity. We blocked gamma-aminobutyric acid-A receptors and cation-chloride cotransport that subtly modulated the low-pH effects on early breathing biorhythms. Further, chloride-free artificial cerebrospinal fluid altered the pattern and timing of breathing biorhythms and blocked the stimulating effect of acidosis in E12-14 brainstems. Early and middle stage embryos exhibited rebound plasticity in brainstem motor outputs during low-pH treatment, which was eliminated by chloride-free solution. Results show that chloride modulates low-pH sensitivity and rebound plasticity in the zebra finch embryonic brainstem, but work is needed to determine the cellular and circuit mechanisms that control functional chloride balance during acid-base disturbances.

UNASSIGNED: The electrochemical dissolution method of instrument retrieval emphasizes on the dissolution of the instrument rather than sacrificing dentine. Most of the studies conducted for electrochemical dissolution used fluoride-containing electrolytes and were performed inside a beaker. In this study, we used chloride-based fluids as electrolytes.
UNASSIGNED: Fifty extracted mandibular first premolars were divided into five groups based on the electrolytes used. Canals were enlarged to ProTaper Universal F2, and files were intentionally broken inside the canal. These specimens were subjected to electrochemical characterization by applying the potential of 9V for 20 min. Optical images were taken to assess the change in surface topography. The results were analyzed statistically by one-way analysis of variance (analysis of variance [ANOVA]).
UNASSIGNED: The rate of dissolution based on the electrolyte used decreased in the following order, viz. Tyrode\'s solution>artificial saliva>normal saline>Ringer\'s lactate/physiological serum.
UNASSIGNED: Apart from fluoride, chloride-based electrolytes could be an efficient alternative.

UNASSIGNED: This study investigated serum, vitreous, and anterior chamber fluid electrolyte changes, corneal thickness (CT), corneal volume (CV), anterior chamber volume (ACV), and anterior chamber depth (ACD) as an auxiliary diagnostic method in the identification of drowning in fresh or salt water.
UNASSIGNED: The study used 35 healthy, adult, male, white New Zealand rabbits, seperated into five groups (control, saltwater drowning (SWD), saltwater immersion (SWI), freshwater drowning (FWD), freshwater immersion (FWI)). CT, CV, ACV, and ACD measurements were made with Pentacam topography at 0, 2, and 4 h in all groups. Magnesium (mg), sodium (Na), and chlorine (Cl) were measured in the blood at 0 and 2 h, and in blood, vitreous fluid, and humor aqueous at 4 h.
UNASSIGNED: It was determined that CT, CV, ACV, and ACD are not of great value in drowning diagnosis and are affected by the fresh or salt water rather than drowning. Vitreous Na, Cl, and Mg levels are ineffective in determining drowning after one h. Anterior chamber fluid may provide valuable information in the differentiation freshwater - saltwater drownings at the 4th h in corpses retrieved from water.
UNASSIGNED: Anterior chamber fluid Na and Cl levels, especially in corpses removed from salt water, can be an easily used test that can help diagnose drowning.

Hydrogen-tuned 185 nm vacuum ultraviolet (VUV/H2) photolysis is an emerging technology to destroy per- and polyfluoroalkyl substance (PFAS) in brine. This study discovered the promotive effects of two major brine anions, i.e., chloride and sulfate in VUV/H2 photolysis on the hydrated electron (eaq-) generation and perfluorocarboxylates (PFCAs) destruction and established a kinetics model to elucidate the promotive effects on the steady-state concentration of eaq- ([eaq-]ss). Results showed that VUV/H2 achieved near-complete defluorination of perfluorooctanoic acid (PFOA) in the presence of up to 1000 mM chloride or sulfate at pH 12. The defluorination rate constant (kdeF) of PFOA peaked with a chloride concentration at 100 mM and with a sulfate concentration at 500 mM. The promotive effects of chloride and sulfate were attributed to an enhanced generation of eaq- via their direct VUV photolysis and conversion of additionally generated hydroxyl radical to eaq- by H2, which was supported by a linear correlation between the predicted [eaq-]ss and experimentally observed kdeF. The kdeF value increased from pH 9 to 12, which was attributed to the speciation of the H·/eaq- pair. Furthermore, the VUV system achieved >95% defluorination and ≥99% parent compound degradation of a concentrated PFCAs mixture in a synthetic brine, without generating any toxic perchlorate or chlorate.

The present study investigated the prevalence of blood chloride (Cl) abnormalities in diarrheic neonatal calves with metabolic acidosis and attempted to identify the most relevant electrolyte abnormality to these abnormalities. A retrospective analysis was conducted on the medical records of 157 diarrheic neonatal calves aged 10.3 ± 4.2 days old with metabolic acidosis. Hypochloremia, normochloremia, and hyperchloremia were observed in 8.9% (14/157), 43.3% (68/157), and 47.8% (68/157), respectively, of diarrheic calves with metabolic acidosis. This distribution remained similar regardless of age (under 8 days or 8 days and older). Furthermore, a multiple logistic regression analysis showed that variations in values for blood sodium [Na (regression coefficients 0.877; 95% confidence interval (CI) 13.977-134.195; P<0.01)], pH (regression coefficients -10.719; 95% CI -19.076- -2.362; P<0.05), and bicarbonate [HCO3- (regression coefficients -0.555; 95% CI -0.820- -0.290; P<0.01)] were associated with blood Cl abnormalities. The present results revealed that blood Na concentrations were more strongly associated with blood Cl concentrations than blood pH and HCO3- values. In the present study, diarrheic calves with hyperchloremia were characterized by normonatremia and extremely severe metabolic acidosis.

UNASSIGNED: In a critically ill patient, when an arterial blood sample is processed on an arterial blood gas (ABG) analyzer, it also measures electrolytes apart from analyzing the blood gases. The turnaround time for ABG analysis is way too less compared to the conventional electrolyte analysis with a serum sample.
UNASSIGNED: This study intends to investigate whether values of electrolytes estimated in arterial blood can substitute the routinely practiced method.
UNASSIGNED: This is a retrospective cross-sectional study. The source of data is patients\' reports of serum electrolytes and ABG analysis from the Clinical Biochemistry laboratory, CIMS Teaching Hospital, Chamarajanagar between January and June 2021. The electrolytes report of 200 patients from whom both arterial and venous blood samples were sent to the Clinical Biochemistry laboratory on the same day and at the same time for analysis were selected. The data was compiled, compared, and correlated using a suitable statistical tool.
UNASSIGNED: The mean and standard deviation of sodium (135.62 ± 5.20 in venous vs 134.08 ± 8.49 in arterial blood), potassium (4.20 ± 0.64 vs 3.80 ± 0.75), and chloride (102.28 ± 4.99 vs 96.33 ± 8.11) were observed. However, when the concordance correlation coefficient and Bland-Altman plot analysis were made there was no agreement between electrolytes analyzed on serum in an autoanalyzer with that of ABG analyzer.
UNASSIGNED: We conclude that the electrolytes measured by a conventional autoanalyzer on a serum sample cannot be replaced by values analyzed on a blood gas analyzer.
UNASSIGNED: Devaki RN, Kasargod P, Roopa Urs AN, Chandrika N. A Retrospective Database Analysis to Investigate if Electrolytes in Venous Blood are Equivalent to the Levels in Arterial Blood. Indian J Crit Care Med 2024;28(5):442-446.

The potassium-chloride co-transporter 2, KCC2, is a neuron-specific ion transporter that plays a multifunctional role in neuronal development. In mature neurons, KCC2 maintains a low enough intracellular chloride concentration essential for inhibitory neurotransmission. During recent years, pathogenic variants in the KCC2 encoding gene SLC12A5 affecting the functionality or expression of the transporter protein have been described in several patients with epilepsy of infancy with migrating focal seizures (EIMFS), a devastating early-onset developmental and epileptic encephalopathy. In this study, we identified a novel recessively inherited SLC12A5 c.692G>A, p. (R231H) variant in a patient diagnosed with severe and drug-resistant EIMFS and profound intellectual disability. The functionality of the variant was assessed in vitro by means of gramicidin-perforated patch-clamp experiments and ammonium flux assay, both of which indicated a significant reduction in chloride extrusion. Based on surface immunolabeling, the variant showed a reduction in membrane expression. These findings implicate pathogenicity of the SLC12A5 variant that leads to impaired inhibitory neurotransmission, increasing probability for hyperexcitability and epileptogenesis.

The cGAS-STING signaling pathway has emerged as a key mediator of inflammation. However, the roles of chloride homeostasis on this pathway are unclear. Here, we uncovered a correlation between chloride homeostasis and cGAS-STING signaling. We found that dysregulation of chloride homeostasis attenuates cGAS-STING signaling in a lysosome-independent manner. Treating immune cells with chloride channel inhibitors attenuated 2\'3\'-cGAMP production by cGAS and also suppressed STING polymerization, leading to reduced cytokine production. We also demonstrate that non-selective chloride channel blockers can suppress the NPC1 deficiency-induced, hyper-activated STING signaling in skin fibroblasts derived from Niemann Pick disease type C (NPC) patients. Our findings reveal that chloride homeostasis majorly affects cGAS-STING pathway and suggest a provocative strategy to dampen STING-mediated inflammation via targeting chloride channels.

Desalinated seawater (DSW) has emerged as a promising solution for irrigation in regions facing water scarcity. However, adopting DSW may impact the existing cultivation model, given the presence of potentially harmful elements, among other factors. A three-year experiment was carried out to assess the short-term effects of four irrigation waters-freshwater (FW), DSW, a mix 1:1 of FW and DSW (MW), and DSW with low boron (B) concentration (DSW-B)-on a \'Rio Red\' grapefruit orchard. These irrigation waters exhibited varying levels of phytotoxic elements, some potentially harmful to citrus trees. Sodium (Na+) and chloride (Cl-) concentrations exceeded citrus thresholds in all treatments, except in DSW-B, whilst B exceeded toxicity levels in DSW and MW treatments. Leaf concentrations of Cl- and Na+ remained low in all treatments, whereas B approached toxic levels only in DSW and MW-irrigated trees. The rapid growth of the trees, preventing excessive accumulation through a dilution effect, protected the plants from significant impacts on nutrition and physiology, such as gas exchange and chlorophyll levels, due to phytotoxic elements accumulation. Minor reductions in photosynthesis in DSW-irrigated trees were attributed to high B in leaves, since Cl- and Na+ remained below toxic levels. The accelerated tree growth effectively prevented the substantial accumulation of phytotoxic elements, thereby limiting adverse effects on tree development and yield. When the maturation of trees reaches maximal growth, the potential accumulation of phytotoxic elements is expected to increase, potentially influencing tree behavior differently. Further study until the trees reach maturity is imperative for comprehensive understanding of the long-term effects of desalinated seawater irrigation.