■对于跟腱断裂(ATR)的非手术治疗越来越感兴趣。然而,非手术治疗受到肌腱断裂风险和低满意率的限制.最近,据报道,注射atelocollagen对肌腱愈合有有益作用。
■评价注射前胶原对跟腱愈合的有益作用,探讨前胶原对腱愈合的作用机制。
■对照实验室研究。
■对66只大鼠进行了右跟腱的经皮肌腱切开术。将动物平均分为非注射组(NG)和胶原注射组(CG)。在第1、3和6周,跟腱功能指数,横截面积,负载失效,刚度,压力,并评估了改良的Bonar评分。透射电子显微镜,西方印迹,和免疫组织化学也进行了。
■跟腱功能指数(分别为-6.8和-43.0;P=.040),失效载荷(42.1对27.0N,分别为;P=.049),和刚度(18.8vs10.3N/mm,分别;P=.049)在3周时,CG高于NG。两组间组织学评分差异无统计学意义。透射电镜分析表明,在3周时,CG中胶原纤维的平均直径大于NG中的胶原纤维的平均直径(117.2vs72.6nm,分别为;P<.001)和6周(202.1vs144.0nm,分别;P<.001)。Westernblot分析表明,在1周(P=.005)和6周(P=.001),CG中I型胶原的表达高于NG中的表达。
■骨胶原注射对非手术治疗的跟腱损伤的愈合具有有益作用。CG大鼠的跟腱表现出更好的功能,生物力学,与NG大鼠的形态学结果比较。分子数据表明,去端胶注射的机制可能与I型胶原蛋白的增加有关。
■去端胶注射可能是非手术治疗ATR的良好辅助选择。
UNASSIGNED: There is growing interest in nonoperative treatment for the management of Achilles tendon ruptures (ATRs). However, nonoperative treatment is limited by the risk of tendon reruptures and low satisfaction rates. Recently,
atelocollagen injections have been reported to have beneficial effects on tendon healing.
UNASSIGNED: To evaluate the beneficial effects of injected
atelocollagen on Achilles tendon healing and investigate the mechanism of
atelocollagen on tendon healing.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: Percutaneous tenotomy of the right Achilles tendon in 66 rats was performed. The animals were equally divided into the noninjection group (NG) and the collagen injection group (CG). At 1, 3, and 6 weeks, the Achilles functional index, cross-sectional area, load to failure, stiffness, stress, and the modified Bonar score were assessed. Transmission electron microscopy, western blotting, and immunohistochemistry were also performed.
UNASSIGNED: The Achilles functional index (-6.8 vs -43.0, respectively; P = .040), load to failure (42.1 vs 27.0 N, respectively; P = .049), and stiffness (18.8 vs 10.3 N/mm, respectively; P = .049) were higher in the CG than those in the NG at 3 weeks. There were no significant differences in histological scores between the 2 groups. Transmission electron microscopy analysis showed that the mean diameter of collagen fibrils in the CG was greater than that in the NG at 3 weeks (117.2 vs 72.6 nm, respectively; P < .001) and 6 weeks (202.1 vs 144.0 nm, respectively; P < .001). Western blot analysis showed that the expression of collagen type I in the CG was higher than that in the NG at 1 week (P = .005) and 6 weeks (P = .001).
UNASSIGNED: An atelocollagen injection had beneficial effects on the healing of nonoperatively treated Achilles tendon injuries. The Achilles tendon of CG rats exhibited better functional, biomechanical, and morphological outcomes compared with NG rats. The molecular data indicated that the mechanism of
atelocollagen injections may be associated with an increased amount of collagen type I.
UNASSIGNED: An
atelocollagen injection might be a good adjuvant option for the nonoperative treatment of ATRs.