含有软骨的仿生支架的研制,钙化软骨,和骨再生精确的骨软骨修复仍然是一个挑战。在这里,一种新型的三层支架,其顶层含有II型atelocolagen和硫酸软骨素,用于软骨再生,具有II型骨胶原和羟基磷灰石的中间层,用于钙化软骨形成,和底层的I型atelocollagen和羟基磷灰石的骨骼生长-可以建立使用液相共合成,被描述。与单层支架相比,三层支架的机械性能明显优越,脱层风险较低。这是由于每个层之间的界面产生的更高的内聚力。体外实验结果表明,尽管单层支架可以刺激骨髓干细胞分化形成软骨,钙化软骨,和骨分别(使用定量聚合酶链反应分析和番红O和茜素红S染色检测),三层支架支持软骨的再生,钙化软骨,在植入2个月和4个月后同时和骨骼(使用肉眼和显微计算机断层扫描图像检测,组织学染色,和Avizo,用于检测金属中微观缺陷的软件)。这项工作提供了一种有希望的方法来设计精确修复骨软骨缺损的策略的数据。
The development of biomimetic scaffolds containing cartilage, calcified cartilage, and bone regeneration for precise osteochondral repair remains a challenge. Herein, a novel tri-layered scaffold-with a top layer containing type II
atelocollagen and chondroitin sulphate for cartilage regeneration, an intermediate layer with type II
atelocollagen and hydroxyapatite for calcified cartilage formation, and a bottom layer with type I
atelocollagen and hydroxyapatite for bone growth-that can be built using liquid-phase cosynthesis, is described. The tri-layered scaffolds are mechanically demonstrably superior and have a lower risk of delamination than monolayer scaffolds. This is due to higher cohesion arising from the interfaces between each layer. In vitro results show that although monolayer scaffolds can stimulate bone marrow stem cells to differentiate and form cartilage, calcified cartilage, and bone separately (detected using quantitative polymerase chain reaction analysis and staining with safranin-O and Alizarin Red S), the tri-layered scaffolds support the regeneration of cartilage, calcified cartilage, and bone simultaneously after 2 and 4 months of implantation (detected using gross and micro-computed tomography images, histological staining, and Avizo, a software used to detect microlevel defects in metals). This work presents data on a promising approach in devising strategies for the precise repair of osteochondral defects.