调查尼日利亚抗生素抗性细菌(ARB)和抗生素抗性基因(ARG)的环境热点的研究有限。这项研究旨在评估各种环境来源和通常接触的表面作为ARB和ARG的潜在携带者,对公共卫生具有影响。共392个样品,包括污水(36),污泥(36),尿布(20),塑料(20),水袋聚乙烯袋(20),食物垃圾(20)垃圾场下的土壤(20),经常接触的表面,如洗手间地板(80),走廊(24)门把手(56),和房间地板和墙壁(60),收集并筛选是否存在携带基因的耐药细菌,如BLAKPC,BLANDM-1,BLACMY-2,BLAIMP,BlaOXA66和MecA.此外,我们采用标准技术检测耐甲氧西林金黄色葡萄球菌(MRSA)和产超广谱β-内酰胺酶(ESBL)的大肠杆菌,肺炎克雷伯菌,铜绿假单胞菌和鲍曼不动杆菌。我们还评估了常规消毒程序在消除洗手间地板上的ARB方面的有效性。我们的研究结果表明,污水,污泥,尿布,食物垃圾和洗手间地板经常被高度和中度耐药的大肠杆菌菌株污染,肺炎克雷伯菌,铜绿假单胞菌和MRSA。值得注意的是,我们在从这些环境来源分离的鲍曼不动杆菌中鉴定了BlaOXA51样基因的两种变体(BlaOXA-66和BlaOXA-180)。此外,我们检测到7个ESBL-肺炎克雷伯菌,五个ESBL-A鲍曼不动杆菌,两个ESBL-大肠杆菌和一个ESBL-铜绿假单胞菌,所有携带一个或多个ARG(blaKPC,BLANDM-1,BLACMY-2),在从污水中回收的分离物中,污泥,洗手间地板和塑料。值得注意的是,即使在消毒程序之后,ARB仍在洗手间地板上。总之,这项研究强调,在居民区和个人共享表面中随意丢弃的环境废物被携带具有重大公共卫生重要性的ARG的ARB大量定居。
Studies investigating environmental hotspots of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Nigeria are limited. This study was designed to assess various environmental sources and commonly touched surfaces as potential carriers of ARB and ARGs with implications for public health. A total of 392 samples, including sewage (36),
sludge (36), diapers (20), plastics (20), water sachet polythene bags (20), food wastes (20), soil beneath dump sites (20), and frequently touched surfaces such as restroom floors (80), corridors (24), door handles (56), and room floors and walls (60), were collected and screened for the presence of resistant bacteria carrying genes such as bla KPC, bla NDM-1, bla CMY-2, bla IMP, bla OXA66 and MecA. Additionally, we employed standard techniques to detect methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii . We also evaluated the effectiveness of routine disinfection procedures in eliminating ARB from restroom floors. Our findings revealed that sewage,
sludge, diapers, food wastes and restroom floors are frequently contaminated with highly and moderately resistant strains of E. coli, K. pneumoniae, P. aeruginosa and MRSA. Notably, we identified two variants of the bla OXA51-like gene (bla OXA-66 and bla OXA-180) in A. baumannii isolated from these environmental sources. Furthermore, we detected seven ESBL- K. pneumoniae , five ESBL- A. baumannii , two ESBL- E. coli and one ESBL- P. aeruginosa , all carrying one or more ARGs (bla KPC, bla NDM-1, bla CMY-2), in isolates recovered from sewage,
sludge, restroom floors and plastics. It is of note that ARB persisted on restroom floors even after disinfection procedures. In conclusion, this study highlights that environmental wastes indiscriminately discarded in residential areas and shared surfaces among individuals are heavily colonized by ARB carrying ARGs of significant public health importance.