背景:齿状回声具有潜在毒性,可引发男性生殖系统的临床损害。山奈酚是一种对蛇毒毒素具有中和潜力的治疗剂。本研究研究了山奈酚对E.ocellatus毒液(EoV)诱导的生殖毒性的拮抗作用。
方法:将50只成年雄性大鼠随机分为5组,每组10只。对照组大鼠分为第1组,第2-5组大鼠腹腔注射EoV0.22mg/kgbw(LD50)。第2组大鼠不治疗,第3-5组大鼠用血清抗蛇毒血清(0.2ml)治疗,envenoming后服用4和8毫克/千克体重的山奈酚,分别。
结果:EoV致生殖毒性,显著降低精子参数,炎症增强,氧化应激,和未治疗的envenomed大鼠生殖器官中的凋亡生物标志物。然而,山奈酚治疗减轻了毒液引起的生殖障碍,具有剂量依赖性。山奈酚显著增加睾丸重量,器官体细胞指数,精子参数,并使血清黄体生成素水平正常化,睾丸激素,和促卵泡激素.山奈酚改善了睾丸和附睾的氧化应激,丙二醛(MDA)水平显着降低,增强还原型谷胱甘肽(GSH)水平,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)活性。炎症生物标志物;一氧化氮(NO)水平和髓过氧化物酶活性(MPO),和凋亡生物标志物;在用山奈酚治疗的大鼠的睾丸和附睾中,caspase3和caspase9的活性被基本抑制。
结论:结果显示山奈酚是一种潜在的治疗生殖毒性的药物,可以在毒蛇后出现。
BACKGROUND: Echis ocellatus envenoming is potentially toxic initiating clinical damages on male reproductive system. Kaempferol is a therapeutic agent with neutralizing potentials on snake venom toxins. This study investigated the antagonistic effect of kaempferol on E. ocellatus venom (EoV)-induced reproductive toxicities.
METHODS: Fifty adult male rats were sorted at random into five groups of ten rats for this study. The control rats were allotted to group 1, while rats in groups 2-5 were injected with 0.22 mg/kg bw (LD50) of EoV intraperitoneally. Rats in group 2 were not treated while groups 3-5 rats were treated with serum antivenom (0.2 ml), and 4 and 8 mg/kg bw of kaempferol post envenoming, respectively.
RESULTS: EoV actuated reproductive toxicity, significantly decreased sperm parameters, and enhanced inflammatory, oxidative stress, and apoptotic biomarkers in reproductive organs of untreated envenomed rats. However, treatment with kaempferol alleviated the venom-induced reproductive disorders with a dose dependent effect. Kaempferol significantly increased the testicular weight, organo-somatic index, sperm parameters, and normalized the levels of serum luteinizing hormone, testosterone, and follicle stimulating hormone. Kaempferol ameliorated testicular and epididymal oxidative stress as evidenced by significant decrease in malondialdehyde (MDA) levels, enhancement of reduced glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The inflammatory biomarkers; nitric oxide (NO) levels and myeloperoxidase activity (MPO), and apoptotic biomarkers; caspase 3 and caspase 9 activities were substantially suppressed in the testis and epididymis of envenomed rats treated with kaempferol.
CONCLUSIONS: Results revealed kaempferol as a potential remedial agent against reproductive toxicity that could manifest post-viper envenoming.