PDF(Pubmed)
Abstract：
Every day, millions of individuals are exposed to formaldehyde (FA) due to its extensive presence and versatile use. Many in vivoand in vitroexperiments revealed that the mechanism of genotoxicity induced by FA exposure is complex yet toxicity upon whole-body exposure (WBE) to FA is less. As teachers, students, and skilled assistants in the health care sectors are also extensively exposed to FA vapors, it might result in genotoxicity. However, the effects of subchronic exposure to FA at low concentrations are not clear. Hence, analysis of the micronucleus (MN) was necessary to study the genetic toxicity triggered by FA in the bone marrow of male and female experimental rats. The present study is a gender- and duration of exposure-based assessment of the geno- and cytotoxicity in bone marrow cells of Wistar rats to study the effect of WBE to 10% FA on polychromatic erythrocytes/normochromatic erythrocytes (PCE/NCE) ratio and micronucleated polychromatic erythrocytes (MnPCE) in experimental rats. The obtained result clearly showed that WBE to FA for 60 days at concentrations between 1 and 1.1 ppm (0, 1, and 1.5 h) induced genotoxic effects in both male and female rats by altering the MnPCE% and significantly increasing the ratio of PCE/NCE (1.07 ± 0.23, 1.20 ± 0.20, 1.22 ± 0.14). The PCE/NCE ratio in male rats was lesser (0.98, 1.12, and 1.18) when compared with female rats (1.17, 1.29, and 1.26) with 0, 1, and 1.5 h exposure, respectively. Thus, the genetic/cellular sensitivity to FA differs among the sexes and also depends on the exposure duration.
摘要：
每一天,由于甲醛的广泛存在和广泛使用，数百万人暴露于甲醛（FA）。许多活体和体外实验表明，FA暴露引起的遗传毒性机制很复杂，但全身暴露（WBE）对FA的毒性较小。作为教师,学生,医疗保健部门的熟练助手也广泛接触FA蒸气，可能会导致遗传毒性.然而,低浓度FA亚慢性暴露的影响尚不清楚.因此，微核（MN）分析对于研究FA在雄性和雌性实验大鼠骨髓中引发的遗传毒性是必要的。本研究是对Wistar大鼠骨髓细胞的遗传和细胞毒性进行基于性别和持续时间的暴露评估，以研究WBE对10％FA对多色红细胞/常色红细胞（PCE/NCE）比率和微核多色红细胞（MnPCE）的影响。获得的结果清楚地表明，浓度在1至1.1ppm（0、1和1.5h）之间的WBE至FA持续60天，通过改变MnPCE％并显着增加PCE/NCE（1.07±0.23，1.20±0.20，1.22±0.14），在雄性和雌性大鼠中均引起基因毒性作用。与0、1和1.5h暴露的雌性大鼠（1.17、1.29和1.26）相比，雄性大鼠的PCE/NCE比更小（0.98、1.12和1.18）。分别。因此，对FA的遗传/细胞敏感性因性别而异，并且还取决于暴露持续时间。
