Tankyrases,聚(ADP-核糖)聚合酶家族中的通用蛋白质组,对翻译后聚(ADP-核糖基)至关重要,影响各种细胞功能并导致疾病,尤其是癌症。因此,tankyrase已成为抗癌药物开发的重要靶标。药物发现中的新兴方法旨在破坏tankyrases与其结合伴侣之间的相互作用,它取决于伴侣蛋白内的tankyrase结合基序(TBM)和tankyrase内的锚蛋白重复簇结构域。我们的研究解决了识别和排名TBM的挑战。我们对现有文献进行了全面回顾,将TBM分为三个不同的组,每个人都有自己的评分系统。为了促进这一进程,我们介绍TBMHunter-anaccessible,基于Web的工具。这个用户友好的平台提供了一种免费且有效的方法来筛选和评估任何给定蛋白质中的潜在TBM。TBMHunter可以同时处理单个蛋白质或蛋白质列表。值得注意的是,我们的结果表明,TBMHunter不仅可以识别已知的TBM,而且还可以发现新的TBM。总之,我们的研究提供了一个全面的角度对TBM,并提出了一个易于使用,精确,以及用于识别和评估任何蛋白质中潜在TBM的免费工具,从而加强专注于tankyrase的研究和药物开发工作。
Tankyrases, a versatile protein group within the poly(ADP-ribose) polymerase family, are essential for post-translational poly(ADP-ribosyl)ation, influencing various cellular functions and contributing to diseases, particularly cancer. Consequently, tankyrases have become important targets for anti-cancer drug development. Emerging approaches in drug discovery aim to disrupt interactions between tankyrases and their binding partners, which hinge on tankyrase-binding motifs (TBMs) within partner proteins and ankyrin repeat cluster domains within tankyrases. Our study addresses the challenge of identifying and ranking TBMs. We have conducted a comprehensive review of the existing literature, classifying TBMs into three distinct groups, each with its own scoring system. To facilitate this process, we introduce TBM Hunter-an accessible, web-based tool. This user-friendly platform provides a cost-free and efficient means to screen and assess potential TBMs within any given protein. TBM Hunter can handle individual proteins or lists of proteins simultaneously. Notably, our results demonstrate that TBM Hunter not only identifies known TBMs but also uncovers novel ones. In summary, our study offers an all-encompassing perspective on TBMs and presents an easy-to-use, precise, and free tool for identifying and evaluating potential TBMs in any protein, thereby enhancing research and drug development efforts focused on tankyrases.