两个革兰氏染色阴性,杆状细菌,命名为菌株KJ10-1T和KJ40-1T,是从海洋褐藻中分离出来的。两株都是过氧化氢酶阳性,氧化酶阳性,和兼性有氧。菌株KJ10-1T在25°C表现出最佳生长,pH7.0和3%NaCl,而菌株KJ40-1T在25℃时表现出最佳生长,pH7.0,和2%NaCl。KJ10-1T菌株的呼吸醌是泛醌-8,泛醌-7,甲基萘醌-7和甲基化甲基萘醌-7,而KJ40-1T菌株的呼吸醌仅是泛醌-8。作为主要的脂肪酸,菌株KJ10-1T含有C16:0,C17:1ω8c,iso-C15:0和求和特征3(C16:1ω7c和/或C16:1ω6c),菌株KJ40-1T包含C16:0和求和特征3和8(C18:1ω7c和/或C18:1ω6c)。菌株KJ10-1T中的主要极性脂质是磷脂酰乙醇胺,磷脂酰甘油,还有一种身份不明的氨基脂质,而KJ40-1T菌株是磷脂酰乙醇胺,磷脂酰甘油,和二磷脂酰甘油。菌株KJ10-1T和KJ40-1T的DNAGC含量分别为42.1和40.8mol%,分别。基于16SrRNA基因序列,菌株KJ10-1T和KJ40-1T表现出与唾液希瓦氏菌MMS16-UL250T(98.6%)和弧菌S-1T(95.4%)最接近的亲缘关系,分别。系统发育分析,基于16SrRNA和92个管家基因,表明该菌株在希瓦氏菌属和弧菌属内形成了不同的系统发育谱系。KJ10-1T菌株与其他希瓦氏菌属物种之间的数字DNA-DNA杂交和直系同源平均核苷酸同一性值,以及在菌株KJ40-1T和其他弧菌之间,低于原核物种划定通常接受的阈值。根据表型,化学分类学,和系统发育数据,菌株KJ10-1T和KJ40-1T代表希瓦氏菌属和弧菌属的新种,分别,为此命名为希瓦氏菌。11月。和弧菌藻类sp。11月。被提议,分别。S.phaeophyticola和V.algarum的类型菌株为KJ10-1T(=KACC22589T=JCM35409T)和KJ40-1T(=KACC22588T=JCM35410T),分别。
Two Gram-stain-negative, rod-shaped bacteria, designated as strains KJ10-1T and KJ40-1T, were isolated from marine brown algae. Both strains were catalase-positive, oxidase-positive, and facultative aerobic. Strain KJ10-1T exhibited optimal growth at 25 °C, pH 7.0, and 3 % NaCl, whereas strain KJ40-1T showed optimal growth at 25 °C, pH 7.0, and 2 % NaCl. The respiratory quinones of strain KJ10-1T were ubiquinone-8, ubiquinone-7, menaquinone-7, and methylated menaquinone-7, while the respiratory quinone of strain KJ40-1T was only ubiquinone-8. As major fatty acids, strain KJ10-1T contained C16 : 0, C17 : 1 ω8c, iso-C15 : 0, and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and strain KJ40-1T contained C16 : 0 and summed features 3 and 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids in strain KJ10-1T were phosphatidylethanolamine, phosphatidylglycerol, and an unidentified aminolipid, whereas those in strain KJ40-1T were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C contents of strains KJ10-1T and KJ40-1T were 42.1 and 40.8 mol%, respectively. Based on 16S rRNA gene sequences, strains KJ10-1T and KJ40-1T exhibited the closest relatedness to Shewanella saliphila MMS16-UL250T (98.6 %) and Vibrio rumoiensis S-1T (95.4 %), respectively. Phylogenetic analyses, based on both 16S rRNA and 92 housekeeping genes, showed that the strains formed distinct phylogenic lineages within the genera Shewanella and Vibrio. Digital DNA-DNA hybridization and orthologous average nucleotide identity values between strain KJ10-1T and other Shewanella species, as well as between strain KJ40-1T and other Vibrio species, were below the thresholds commonly accepted for prokaryotic species delineation. Based on the phenotypic, chemotaxonomic, and phylogenetic data, strains KJ10-1T and KJ40-1T represent novel species of the genera Shewanella and Vibrio, respectively, for which the names Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov. are proposed, respectively. The type strains of S. phaeophyticola and V. algarum are KJ10-1T (=KACC 22589T=JCM 35409T) and KJ40-1T (=KACC 22588T=JCM 35410T), respectively.