近年来,光热疗法(PTT)作为一种新兴的癌症治疗方式获得了相当大的兴趣。放射治疗(RT)作为一种传统的治疗方法已广泛应用于临床。然而,RT和PTT治疗受到副作用和穿透深度的限制,分别。此外,肿瘤内的缺氧会导致对治疗的抵抗力增加。
■我们通过调节反应条件合成了多种尺寸的AuPt。选择最小尺寸的AuPt并用叶酸(FA)修饰用于PTT和RT协同治疗。各种方法,包括透射电子显微镜(TEM),X射线光电子能谱(XPS),X射线衍射(XRD)和傅里叶变换红外光谱(FITR)用于确定AuPt-FA(AF)的结构和组成。此外,我们用红外相机和红外激光器研究了AF的光热特性。流式细胞术,集落形成试验,CCK8,并进行荧光染色以探查体外处理效果。此外,我们通过TEM和体内成像系统(IVIS)探索了AF的靶向性。体内实验,我们记录肿瘤体积和重量的变化以及肿瘤切片的染色(ROS,Ki67和苏木精和伊红)。
与其他尺寸的AuPt(30nm和100nm)相比,粒径为16nm的AuPt赋予其显著高的光热转化效率(46.84%)和过氧化氢酶活性。AF缓解肿瘤微环境中的缺氧,导致在治疗过程中产生更多的活性氧(ROS)。此外,联合RT和PTT治疗效果显着增强,体外凋亡率为81.1%,体内肿瘤体积缩小率为94.0%。
■这些结果表明,AF增强了PTT和RT的协同作用,并具有临床转化的潜力。
UNASSIGNED: Photothermal therapy (PTT) has gained considerable interest as an emerging modality for cancer treatment in recent years. Radiation therapy (RT) has been widely used in the clinic as a traditional treatment method. However, RT and PTT treatments are limited by side effects and penetration depth, respectively. In addition, hypoxia within the tumor can lead to increased resistance to treatment.
UNASSIGNED: We synthesized multiple sizes of AuPt by modulating the reaction conditions. The smallest size of AuPt was selected and modified with folic acid (FA) for PTT and RT synergy therapy. Various methods including transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FITR) are used to determine the structure and composition of AuPt-FA (AF). In addition, we researched the photothermal properties of AF with IR cameras and infrared lasers. Flow cytometry, colony formation assays, CCK8, and fluorescent staining for probing the treatment effect in vitro. Also, we explored the targeting of AF by TEM and In Vivo Imaging Systems (IVIS). In vivo experiments, we record changes in tumor volume and weight as well as staining of tumor sections (ROS, Ki67, and hematoxylin and eosin).
UNASSIGNED: The AuPt with particle size of 16 nm endows it with remarkably high photothermal conversion efficiency (46.84%) and catalase activity compared to other sizes of AuPt (30 nm and 100 nm). AF alleviates hypoxia in the tumor microenvironment, leading to the production of more reactive oxygen species (ROS) during the treatment. In addition, the therapeutic effect was significantly enhanced by combining RT and PTT, with an apoptosis rate of 81.1% in vitro and an in vivo tumor volume reduction rate of 94.0% in vivo.
UNASSIGNED: These results demonstrate that AF potentiates the synergistic effect of PTT and RT and has the potential for clinical translation.