谷子壳色泽是反映谷子某些营养品质参数的重要指标。通过杂交黄壳品种Yugu5和棕色壳品种Jigu31形成的F2:6重组自交系(RIL)种群,用于确定船体颜色性状的遗传控制。该种群以2:1的比例隔离黄色和棕色船体颜色,表明船体颜色受多个遗传基因座调节。使用来自30个具有棕色和黄色外壳颜色的品系的RNA块进行的批量分离分析-RNA测序(BSR-Seq)方法,每个确定了1号染色体上的三个基因组区域(4,570,517-10,698,955bp),2(40,301,380-46,168,003个基点),和3(44,469,860-50,532,757个基点)。吉谷31号棕色船体颜色QHC的新QTL。czas1是在1号染色体上的bin标记Block43和Block697之间检测到的,其遗传连锁图谱是通过对147个RIL的子集进行重新测序而构建的。该QTL解释了从28.0%到47.0%的高水平表型变异。谷子参考基因组中该QTL的相应基因组区域与BSR-Seq分析在1号染色体上检测到的区域重叠。在该基因组区域注释了19个与花色苷生物合成相关的基因。编码SANT/Myb结构域蛋白的基因Si1g06530在发育中的穗和种子中高表达,这是值得进一步验证的候选基因为棕色船体的吉谷31。此外,在2号和3号染色体的基因组区域中鉴定了几个用于花青素生物合成的注释基因。
Hull color of foxtail millet is an important indicator of certain nutritional quality parameters. An F2:6 recombinant inbred line (RIL) population developed by crossing a yellow-hulled cultivar Yugu 5 and a brown-hulled cultivar Jigu 31 was used to determine the genetic control of the hull color trait. This population segregated for yellow and brown hull colors in a ratio of 2:1, indicating that hull color is regulated by multiple genetic loci. A bulk segregant analysis-RNA sequencing (BSR-Seq) approach performed using the RNA bulks from 30 lines with brown and yellow hull colors each identified three genomic regions on chromosomes 1 (4,570,517-10,698,955 bp), 2 (40,301,380-46,168,003 bp), and 3 (44,469,860-50,532,757 bp). A new QTL for brown hull color of Jigu 31, QHC.czas1, was detected between bin markers Block43 and Block697 on chromosome 1 with the genetic linkage map constructed by re-sequencing a subset of the 147 RILs. This QTL explained a high level of phenotypic variation ranging from 28.0% to 47.0%. The corresponding genomic region of this QTL in the foxtail millet reference genome overlapped with that detected on chromosome 1 by the BSR-Seq analysis. Nineteen genes associated with biosynthesis of anthocyanin were annotated in this genomic region. Gene Si1g06530 encoding a SANT/Myb domain protein was highly expressed in developing panicles and seeds, which warrants further verification as the candidate gene for the brown color hull of Jigu 31. Moreover, several annotated genes for biosynthesis of anthocyanin were identified in the genomic regions of chromosomes 2 and 3.