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Abstract：
Low temperature and cold damage are natural factors that seriously reduce wheat yield. Thus, how to improve the cold resistance of wheat has been the focus of wheat breeders and geneticists. However, the genetic improvement for this trait has been slow, mainly because cold resistance is a complex quantitative trait and field phenotypic identification is relatively difficult. Therefore, the discovery, mapping, and cloning of the cold resistance genes of wheat provide a theoretical basis for the genetic improvement of wheat against cold resistance and facilitate the analysis of the molecular mechanisms of cold resistance in wheat. This study used the wheat line H261 and its EMS mutants LF2099 and XiNong 239 as materials. Cold trait segregation occurred in the F2 generation of mutants LF2099 and XiNong 239 at a 15:1 separation ratio. Genetic analysis showed that two dominant overlapping genes, temporarily named Wcr-3 and Wcr-4, control cold resistance in wheat. Furthermore, a combined BSA and SNP array established that Wcr-3 is between BU100519 (SSR marker) and AX-94843669 (SNP marker). The markers are 1.32 cM apart, corresponding to the 5.41 Mb physical interval on the Chinese Spring 2B chromosome with 67 functionally annotated genes. Wcr-4 is located between AX-94657955 (SNP marker) and LC-23 (SSR marker), which are 1.79 cM apart, corresponding to a 2.35 Mb physical interval on the Chinese Spring 2D chromosome, which contains 66 functionally annotated genes. Wcr-3 and Wcr-4 are two new cold resistance genes, laying the foundation for their fine mapping and cloning.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-023-01425-w.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-023-01425-w.
摘要：
低温和冷害是严重降低小麦产量的自然因素。因此，如何提高小麦的抗寒性一直是小麦育种家和遗传学家关注的焦点。然而,这种性状的遗传改进一直很缓慢，主要是因为抗寒性是一个复杂的数量性状，田间表型鉴定相对困难。因此,发现,映射,小麦抗寒基因的克隆为小麦抗寒的遗传改良提供了理论依据，并有助于分析小麦抗寒的分子机制。本研究以小麦品系H261及其EMS突变体LF2099和XiNong239为材料。突变体LF2099和XiNong239的F2代分离以15：1的比例发生了冷性状分离。遗传分析表明,两个显性重叠基因,暂时命名为Wcr-3和Wcr-4，控制小麦的抗寒性。此外，组合的BSA和SNP阵列确定Wcr-3在BU100519(SSR标记)和AX-94843669(SNP标记)之间。标记相距1.32cM，对应于中国春季2B染色体上的5.41Mb物理间隔，具有67个功能注释基因。Wcr-4位于AX-94657955（SNP标记）和LC-23（SSR标记）之间，相距1.79cM，对应于中国春季2D染色体上的2.35Mb物理间隔，其中包含66个功能注释的基因。Wcr-3和Wcr-4是两个新的抗寒基因,为他们精细的作图和克隆奠定了基础。
■在线版本包含补充材料，可在10.1007/s11032-023-01425-w获得。
■在线版本包含补充材料，可在10.1007/s11032-023-01425-w获得。
