PDF(Pubmed)
Abstract：
Fruit peel color is a major factor that influences fruit quality and customers\' demand. However, the molecular mechanisms underlying the green fruit peel color trait of Cucurbita pepo L. remain unknown. Two parental lines, RP16 and RP38, were used to study the fruit peel color trait in C. pepo. The parental line RP16 shows white peel color, whereas RP38 exhibits green peel color. 384 F2 populations were used to identify the inheritance pattern associated with green fruit and white fruit peel in Cucurbita pepo L. 293 F2 individuals were white, and 91 F2 individuals were green, resulting in a ratio of 3:1. Hence, white peel is dominant over the green fruit peel trait, and a single recessive green peel gene (Cpgp) controls the green fruit peel. The fruit chlorophyll (Chll) content decreases as fruit matures in the RP16 line. In contrast, Chll increases during the fruit growing periods on fruit peels of the RP38 line. The BSA-sequence analysis revealed the Cpgp locus on Chr5, within a 2.3 Mb region. Subsequent fine-mapping analysis, using 699 F2 plants, narrowed down this region to 23.90 kb on the same chromosome. Within this region, two annotated genes, namely Cp4.1LG05g02070 and Cp4.1LG05g02060, are present. These genes are predicted to encode a two-component Arabidopsis Pseudo-Response Regulator 2-like protein (APRR2), which may be involved in green pigmentation processes in plants. Consequently, sequence alignment and gene expression analyses at various fruit development stages supported that Cp4.1LG05g02070 may be the primary candidate gene responsible for regulating the green fruit peel color trait in Cucurbita pepo L. This study may provide a basis for further study on the basic mechanisms that control the fruit peel colors in Cucurbita spp.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01492-7.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-024-01492-7.
摘要：
果皮颜色是影响水果品质和顾客需求的主要因素。然而,西葫芦绿色果皮颜色性状的分子机制尚不清楚。两条家长线，RP16和RP38用于研究C.pepo的果皮颜色性状。亲本系RP16显示白色果皮颜色，而RP38表现出绿色的果皮颜色。384个F2种群用于鉴定南瓜中与绿色果实和白色果皮相关的遗传模式。293个F2个体为白色，91个F2个体是绿色的，导致比例为3:1。因此，白色果皮在绿色果皮性状上占主导地位，和单个隐性绿皮基因(Cpgp)控制绿色果皮。在RP16品系中，随着果实的成熟，果实叶绿素（Chll）含量降低。相比之下,在RP38品系的果皮上，Chll在果实生长期增加。BSA序列分析揭示了Chr5上2.3Mb区域内的Cpgp基因座。随后的精细映射分析，使用699个F2植物，将该区域缩小到同一染色体上的23.90kb。在这个区域内,两个注释基因，即Cp4.1LG05g02070和Cp4.1LG05g02060存在。预测这些基因编码两组分拟南芥假反应调节因子2样蛋白（APRR2），这可能与植物的绿色色素沉着过程有关。因此,在各个果实发育阶段的序列比对和基因表达分析支持Cp4.1LG05g02070可能是负责调节葫芦绿色果皮颜色性状的主要候选基因。这项研究可能为进一步研究控制南瓜果皮颜色的基本机制提供基础。
■在线版本包含补充材料，可在10.1007/s11032-024-01492-7获得。
■在线版本包含补充材料，可在10.1007/s11032-024-01492-7获得。
