■类风湿性关节炎(RA)是一种影响人体的慢性自身免疫性疾病。Periplocaforrestii是中国的一种苗族药物,用于治疗关节炎已有数百年的历史。但是,到目前为止,治疗机制尚不清楚。因此,用HPLC-QTOFMS研究了百事可乐对大鼠关节炎的化学成分和作用,Micro-CT,以及本文的其他实验。
雄性Sprague-Dawley大鼠用于评估体内活性。HPLC使用QTOF-MS来分析福雷西氏菌(PF)的化学概况。用牛II型胶原和完全弗氏佐剂刺激并构建胶原诱导的关节炎(CIA)模型。三种剂量的PF(100mg/kg,200mg/kg,400mg/kg)用于评估体内活性。甲氨蝶呤用作阳性药物。采用H/E染色和micro-CT方法监测CIA大鼠的病理变化。ELISA法检测血清免疫和炎症相关细胞因子水平。免疫组织化学实验用于检测JAK和NF-κB途径中的基因表达。
■从PF中鉴定出42种化合物。与对照组和MTX组相比,PF给药降低了脾指数的增加。部分恢复了体重,减少爪子肿胀,关节炎评分与模型组比较。宏观评估显示模型组爪子发炎,肿胀明显,而MTX和PF均减轻了炎症和肿胀的程度。H/E染色实验证明模型组滑膜细胞病理变化及炎性细胞浸润。相比之下,MTX和PF治疗部分逆转了这些病理变化。Micro-CT检查显示模型组严重损伤和炎症引起的疤痕,在高剂量组(400mg/kg)中,炎症引起的损伤和疤痕得到了显着改善。机制研究表明,与模型组相比,PF恢复了NF-κB磷酸化和JAK2表达。
■P.forrestii对CIA大鼠具有有效作用。NF-κB和JAK2通路参与其对CIA的保护作用。
UNASSIGNED: Rheumatoid arthritis (RA) is a chronic autoimmune disease that affects the body. Periploca forrestii was a miao ethnic drug in China that was used to treat arthritis for hundreds of years. But, the therapeutic mechanism is so far unknown. Therefore, the chemical component and effect of Periploca forrestii on arthritis in rats were studied using HPLC-QTOF MS, micro-CT, and other experiments in this paper.
UNASSIGNED: Male Sprague-Dawley rats were used to assess the in vivo activity. HPLC QTOF-MS was used to analyze the chemical profile of the P. forrestii (PF). Bovine type II collagen and Complete Freund\'s Adjuvant were used to stimulate and construct the collagen-induced arthritis (CIA) model. Three dosages of PF (100 mg/kg, 200 mg/kg, 400 mg/kg) were used to evaluate in vivo activity. Methotrexate was used as the positive drug. H/E staining and micro-CT methods were used to monitor the pathological changes of CIA rats. ELISA method was used to assess the serum level of immune- and inflammation-related cytokines. Immunohistochemical experiments were used to test the gene expression in JAK and Nf-κB pathways.
UNASSIGNED: 42 compounds were identified from PF. PF administration lowered the increased spleen index compared with that of control and MTX groups, and partially restored body weight, reduced paw swelling, and arthritis score compared with the model group. Macroscopic assessment indicated inflamed paw with significant swelling in the model group, while the extent of inflammation and swelling was attenuated by both MTX and PF. H/E staining experiments demonstrated that pathological changes of synovial cells and infiltration of inflammatory cells were observed in the model group. In contrast, the MTX and PF treatment partially reversed these pathological changes. Micro-CT examination showed severe injuries and scars caused by inflammation for the model group, and in the high-dosage group (400 mg/kg) the inflammation-caused injuries and scars were dramatically ameliorated. Mechanism study showed that PF restored Nf-κB phosphorylation and JAK2 expression compared with the model group.
UNASSIGNED: P. forrestii possesses a potent effect on CIA rats. Nf-κB and JAK2 pathways are involved in its protective effect on CIA.