PDF(Pubmed)
Abstract：
Aortic aneurysms, life-threatening and often undetected until they cause sudden death, occur when the aorta dilates beyond 1.5 times its normal size. This study used ultrasound scans and micro-computed tomography to monitor and measure aortic volume in preclinical settings, comparing it to the well-established measurement using ultrasound scans. The reproducibility of measurements was also examined for intra- and inter-observer variability, with both modalities used on 8-week-old C57BL6 mice. For inter-observer variability, the μCT (micro-computed tomography) measurements for the thoracic, abdominal, and whole aorta between observers were highly consistent, showing a strong positive correlation (R2 = 0.80, 0.80, 0.95, respectively) and no significant variability (p-value: 0.03, 0.03, 0.004, respectively). The intra-observer variability for thoracic, abdominal, and whole aorta scans demonstrated a significant positive correlation (R2 = 0.99, 0.96, 0.87, respectively) and low variability (p-values = 0.0004, 0.002, 0.01, respectively). The comparison between μCT and USS (ultrasound) in the suprarenal and infrarenal aorta showed no significant difference (p-value = 0.20 and 0.21, respectively). μCT provided significantly higher aortic volume measurements compared to USS. The reproducibility of USS and μCT measurements was consistent, showing minimal variance among observers. These findings suggest that μCT is a reliable alternative for comprehensive aortic phenotyping, consistent with clinical findings in human data.
摘要：
主动脉瘤,危及生命，通常在导致猝死之前未被发现，当主动脉扩张超过其正常大小的1.5倍时发生。这项研究使用超声扫描和显微计算机断层扫描来监测和测量临床前设置的主动脉容积，将其与使用超声波扫描的完善的测量进行比较。还检查了测量结果的可重复性，以了解观察者内部和观察者之间的变异性，两种方式都用于8周龄C57BL6小鼠。对于观察者间的可变性，胸部的μCT（微型计算机断层扫描）测量，腹部，观察者之间的整个主动脉高度一致，显示出强的正相关（R2分别为0.80、0.80、0.95），并且没有显着变异性（p值分别为0.03、0.03、0.004）。胸部观察者内部的变异性，腹部，和整个主动脉扫描显示出显着的正相关（R2分别为0.99，0.96，0.87）和低变异性（p值分别为0.0004，0.002，0.01）。肾上和肾下主动脉的μCT和USS（超声）之间的比较没有显着差异（p值分别为0.20和0.21）。与USS相比,μCT提供显著更高的主动脉容积测量值。USS和μCT测量的可重复性是一致的，观察者之间的差异最小。这些结果表明，μCT是一个可靠的替代综合主动脉表型，与人类数据中的临床发现一致。
