PDF(Pubmed)
Abstract：
The extraction of museum DNA from a unique collection of samples of the \"Crocidura pergrisea\" species complex, which comprises local endemics of Central and West Asia, allowed us to determine their inter- and intragroup relationships. The first step of this study was the re-evaluation of heavily damaged type specimens of C. armenica via a microcomputed-tomography-based cybertaxonomic approach (CTtax), which enabled a precise description of the species\' morphology; three-dimensional models of the cybertypes were made available through the MorphoBank Repository. We developed the \"AProMaDesU\" pipeline on the basis of five requirements for micro-CT-based cyber-datasets in relation to mammalian collections. Our second step was a combination of several meticulous approaches to morphological investigation against a background of a cytb-based phylogeny, which helped us to make a taxonomic decision about the status of species of the \"pergrisea\" group, e.g., C. arispa, C. armenica, and C. serezkyensis, when the morphological results were partly incongruent with the molecular phylogeny. Nevertheless, under two assumptions, our findings preserved a separate species-level status of C. serezkyensis and C. arispa. In addition, we restored the species-level status of C. armenica. This taxonomic decision is based on our morphospace analysis, which revealed unique craniomandibular shape transformations within the rocky shrews that helped them with the transition to a new area of morphospace/trophic niches and consequently separated them from the other analyzed Crocidura groups.
摘要：
从独特的“虎尾草”物种群样本中提取博物馆DNA，包括中亚和西亚的当地地方病，允许我们确定他们的群体间和群体内关系。这项研究的第一步是通过基于微型计算机断层扫描的网络分类学方法（CTtax）重新评估严重受损的C.armenica类型标本。这使得能够精确描述物种形态；网络类型的三维模型可通过MorphoBank存储库获得。我们根据与哺乳动物集合相关的基于micro-CT的网络数据集的五个要求开发了“AProMadesU”管道。我们的第二步是结合基于cytb的系统发育的背景进行形态学研究的几种细致方法，这有助于我们对“pergrisea”组物种的地位做出分类学决定，例如，C.阿里斯帕,C.Armenica,和C.serezkyensis，当形态学结果与分子系统发育部分不一致时。然而，在两个假设下，我们的发现保留了C.serezkyensis和C.arispa的单独物种水平。此外,我们恢复了Armenica的物种水平。这个分类决定是基于我们的形态空间分析，揭示了岩石the中独特的颅骨下颌形状转变，帮助他们过渡到形态空间/营养壁龛的新区域，从而将它们与其他分析的Crocidura组分开。
