植物病原性镰刀菌对作物健康和土壤质量构成重大威胁。虽然我们的实验室培养的假单胞菌。P13表现出潜在的生物控制能力,其对抗禾谷镰刀菌的有效性和潜在的抗真菌机制尚不清楚.鉴于此,我们的研究调查了P13在体外和土壤环境中对F.graminearumT1的显着抑制作用。进行基因组,代谢组学,和P13的转录组学分析,我们试图找到支持其对T1拮抗作用的证据。结果揭示了一种新的假单胞菌属物种P13的潜力,产生活性抗真菌成分,包括吩嗪-1-羧酸盐(PCA),氰化氢(HCN),和铁载体[pyoverdine(Pvd)和histifrusgatin(Hcs)],以及与这些活性成分相关的P13代谢途径的动态适应性变化。在对数生长阶段,T1暴露的P13策略性上调PCA和HCN生物合成,以及对三羧酸(TCA)循环的瞬时抑制。然而,随着增长的稳定,PCA和HCN合成的上调停止,而TCA循环增强,增加铁载体分泌(Pvd和Hcs),提示这种机制可能导致T1的持续抑制。这些发现提高了我们对P13生物防治机制的理解,并为假单胞菌菌株在植物病原性F.的生物防治中的潜在应用奠定了基础。
目的:假单胞菌属。产生各种抗真菌物质,使其成为对抗病原真菌的有效天然生物防治剂。然而,假单胞菌的抑制作用和相关的拮抗机制。对抗镰刀菌。不清楚。新型假单胞菌的体外抗真菌作用的多组学整合分析,P13,对F.graminearumT1揭示了P13产生抗真菌成分的能力(PCA,HCN,Pvd,和Hcs),在对数生长期战略性上调PCA和HCN生物合成,并在固定生长期增强TCA周期。这些发现增进了我们对P13的生物防治机制及其对病原真菌的潜在应用的理解。
Phytopathogenic Fusarium graminearum poses significant threats to crop health and soil quality. Although our laboratory-cultivated Pseudomonas sp. P13 exhibited potential biocontrol capacities, its effectiveness against F. graminearum and underlying antifungal mechanisms are still unclear. In light of this, our study investigated a significant inhibitory effect of P13 on F. graminearum T1, both in vitro and in a soil environment. Conducting genomic, metabolomic, and transcriptomic analyses of P13, we sought to identify evidence supporting its antagonistic effects on T1. The results revealed the potential of P13, a novel Pseudomonas species, to produce active antifungal components, including phenazine-1-carboxylate (PCA), hydrogen cyanide (HCN), and siderophores [pyoverdine (Pvd) and histicorrugatin (Hcs)], as well as the dynamic adaptive changes in the metabolic pathways of P13 related to these active ingredients. During the logarithmic growth stage, T1-exposed P13 strategically upregulated PCA and HCN biosynthesis, along with transient inhibition of the tricarboxylic acid (TCA) cycle. However, with growth stabilization, upregulation of PCA and HCN synthesis ceased, whereas the TCA cycle was enhanced, increasing siderophores secretion (Pvd and Hcs), suggesting that this mechanism might have caused continuous inhibition of T1. These findings improved our comprehension of the biocontrol mechanisms of P13 and provided the foundation for potential application of Pseudomonas strains in the biocontrol of phytopathogenic F. graminearum.
OBJECTIVE: Pseudomonas spp. produces various antifungal substances, making it an effective natural biocontrol agent against pathogenic fungi. However, the inhibitory effects and the associated antagonistic mechanisms of Pseudomonas spp. against Fusarium spp. are unclear. Multi-omics integration analyses of the in vitro antifungal effects of novel Pseudomonas species, P13, against F. graminearum T1 revealed the ability of P13 to produce antifungal components (PCA, HCN, Pvd, and Hcs), strategically upregulate PCA and HCN biosynthesis during logarithmic growth phase, and enhance the TCA cycle during stationary growth phase. These findings improved our understanding of the biocontrol mechanisms of P13 and its potential application against pathogenic fungi.