由于化肥的过度使用对生物体造成伤害,对土壤环境产生不利影响,用生物肥料代替化学肥料作为一种环境保护策略引起了广泛关注。在这项研究中,根际细菌接种对樟子松生长的影响。蒙古幼苗,土壤参数,土壤微生物群落结构,并通过盆栽实验研究了阻尼的生物防治。结果表明,所有三种根际细菌(Pseudomonaschororaphis,极端假单胞菌,和LwoffiiA07不动杆菌)测试显示出生长促进特性,例如吲哚-3-乙酸的生产,水解酶,铁载体,和氰化氢;固氮;和磷溶解。三种细菌的应用增加了植物生物量,根结构,和养分含量,也增加了土壤养分含量和酶活性。细菌接种通过调节土壤的理化性质,促进有益菌和拮抗菌的生长,从而改善细菌群落结构。在土壤特征中,可用氮气,总氮,有效钾,脲酶活性是主要影响因素。此外,还发现细菌接种显着增加了植物超氧化物歧化酶的活性,过氧化氢酶,过氧化物酶,和其他防御酶;增强植物抗病性;有效抑制阻尼;促进植物生长。总之,三种根际细菌的应用系统地影响了植物之间的相互作用,土壤参数,和土壤微生物群落。这些结果为理解根际细菌如何促进樟子树的生长提供了基础。蒙古,从而提供了一个有前途的可持续替代化学肥料。
As the excessive use of chemical fertilizers harms organisms and adversely affects the soil environment, the replacement of chemical fertilizers with biological fertilizers has attracted widespread attention as an environmental protection strategy. In this study, the effects of rhizosphere bacteria inoculation on growth of Pinus sylvestris var. mongolica seedlings, soil parameters, soil microbial community structure, and the biocontrol of damping-off were studied by pot experiments. The results showed that all three rhizosphere bacteria (Pseudomonas chlororaphis, Pseudomonas extremaustralis, and Acinetobacter lwoffii A07) tested exhibited growth-promoting properties, such as the production of indole-3-acetic acid, hydrolase, siderophores, and hydrogen cyanide; nitrogen fixation; and phosphorus solubilization. The application of the three bacteria increased plant biomass, root structure, and nutrient content and also increased soil nutrient content and enzyme activity. Bacterial inoculation promoted the growth of beneficial bacteria and antagonistic bacteria by adjusting the physicochemical properties of the soil, thereby improving the bacterial community structure. Among the soil features, available nitrogen, total nitrogen, available potassium, and urease activity were the main influencing factors. In addition, it was also found that bacterial inoculation significantly increased the activities of plant superoxide dismutase, catalase, peroxidase, and other defense enzymes; enhanced plant disease resistance; effectively inhibited damping-off; and promoted plant growth. In summary, the application of three rhizosphere bacteria systematically affected the interaction between plants, soil parameters, and soil microbial communities. These results provide a basis for understanding how rhizosphere bacteria promote the growth of P. sylvestris var. mongolica, thereby offering a promising sustainable alternative to chemical fertilizers.