背景:海洛因使用障碍(HUD)是一个日益严重的健康问题,占药物滥用者死亡的大多数。研究药物滥用者的非编码核糖核酸基因表达是一种有前途的方法,因为它可以用于诊断和治疗。
方法:从KasrAlAiny精神病学和成瘾门诊诊所招募了49名男性海洛因依赖患者和49名男性对照参与者,医学院,开罗大学。Sera被收集。qRT-PCR用于检测非编码RNA的基因表达,例如“HOX转录本反义RNA”(HOTAIR),micro-RNA(miRNA-206),磷脂酰肌醇3-激酶(PI3K),蛋白激酶B(AKT),雷帕霉素的机械靶(mTOR),和活性调节的细胞骨架相关蛋白(Arc)。使用ELISA评估血清脑源性神经营养因子(BDNF)水平。使用蛋白质印迹可以确定PI3K的蛋白质表达,AKT,还有MTOR.
结果:研究表明,HOTAIR的基因表达,AKT,PI3K,病例和对照组之间的电弧大大降低,miR-206和mTOR1的基因表达显著升高。PI3K和AKT蛋白表达下调,而mTOR表达上调。BDNF水平在某些情况下显著降低。
结论:这项研究的结果表明,HUD中HOTAIR的降低减轻了miR-206的抑制,从而增加并影响下游PI3K/AKT/mTOR,ARC,和BDNF表达。这可能在成瘾和复发行为中共享。
BACKGROUND: Heroin use disorder (HUD) is a seriously increasing health issue, accounting for most deaths among drug abusers. Studying non-coding ribonucleic acid gene expression among drug abusers is a promising approach, as it may be used in diagnosis and therapeutics.
METHODS: A total of 49 male heroin-dependent patients and 49 male control participants were recruited from Kasr Al Ainy Psychiatry and Addiction outpatient clinics, Faculty of Medicine, Cairo University. Sera were gathered. qRT-PCR was utilized for the detection of gene expression of non-coding RNAs such as \"HOX transcript antisense RNA\" (HOTAIR), micro-RNA (miRNA-206), phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), mechanistic target of rapamycin (mTOR), and Activity Regulated Cytoskeleton Associated Protein (Arc). Sera Brain-Derived Neurotrophic Factor (BDNF) levels were assessed using ELISA. Using a western blot made it possible to determine the protein expression of PI3K, AKT, and mTOR.
RESULTS: The study demonstrated that gene expressions of HOTAIR, AKT, PI3K, and Arc were considerably lowered between cases and controls, while gene expressions of miR-206 and mTOR1 were significantly raised. PI3K and AKT protein expressions were downregulated, while mTOR expressions were upregulated. BDNF levels were significantly decreased in some cases.
CONCLUSIONS: The results of this study suggest that decreased HOTAIR in HUD relieves miR-206 inhibition, which thus increases and affects downstream PI3K/AKT/mTOR, ARC, and BDNF expression. This may be shared in addictive and relapsing behaviors.