本研究的目的是确定与单独的发色团/光处理相比,同时存在的核黄素/UV-A光(RF/UV-A)和玫瑰红/绿光(RB/绿光)PACK-CXL是否增强角膜对酶消化的抗性。
■离体猪角膜分配如下。A组角膜用核黄素(RF)浸泡,不照射(A1,对照)或用10(A2)或15J/cm²(A3)UV-A光照射365nm,分别。B组角膜用RB浸泡,未照射(B1,对照)或在525nm下用10(B2)或15J/cm²(B3)绿光照射,分别。C组中的角膜用RF和RB浸泡,并且不进行辐照(C1,对照)或连续10J/cm2(C2)或15J/cm2(C3)UV-A和绿光暴露。治疗后,所有角膜均暴露于0.3%胶原酶A以评估消化时间直至角膜纽扣溶解.
■A1至A3消化时间分别为21.38、30.5和32.25小时,分别,A2和A3对A1的抗性增加。B1-3的消化时间为31.2、33.81和34.38小时,B3抵抗超过B1。C1至C3时间分别为33.47、39.81和51.94小时;C3对C1和C2表现出较好的抗性(均P<0.05)。
■与单独治疗相比,相同的RF/UV-A和RB/绿色PACK交联技术显着增加了角膜酶消化抗性。
■结合基于RF和基于RB的PACK-CXL可显著提高角膜胶原酶的消化抗性,在临床环境中可能最小化溃疡大小。
The purpose of this study was to determine if concurrent riboflavin/UV-A light (RF/UV-A) and rose Bengal/green light (RB/green) epi-off PACK-CXL enhances corneal resistance to enzymatic digestion compared to separate chromophore/light treatments.
Ex vivo porcine corneas were allocated as follows. Group A corneas were soaked with riboflavin (RF) and were either not irradiated (A1, controls) or were irradiated with 10 (A2) or 15 J/cm² (A3) UV-A light at 365 nm, respectively. Group B corneas were soaked with RB and either not irradiated (B1, controls) or were illuminated with 10 (B2) or 15 J/cm² (B3) green light at 525 nm, respectively. Corneas in group C were soaked with both RF and RB and were either not irradiated (C1, controls) or were subjected to the same session consecutive 10 J/cm2 (C2) or 15 J/cm2 (C3) UV-A and green light exposure. Following treatment, all corneas were exposed to 0.3% collagenase A to assess digestion time until corneal button dissolution.
A1 to A3 digestion times were 21.38, 30.5, and 32.25 hours, respectively, with A2 and A3 showing increased resistance to A1. B1-3 had digestion times of 31.2, 33.81, and 34.38 hours, with B3 resisting more than B1. C1 to C3 times were 33.47, 39.81, and 51.94 hours; C3 exhibited superior resistance to C1 and C2 (both P < 0.05).
Same-session combined RF/UV-A and RB/green PACK-cross-linking significantly increases corneal enzymatic digestion resistance over standalone treatments.
Combining RF-based and RB-based PACK-CXL considerably increases corneal collagenase digestion resistance, potentially minimizing ulcer size in clinical contexts.