UASSIGNED:光疗是治疗新生儿高胆红素血症的推荐方法。然而,缺乏用于预测治疗前光疗更有效持续时间的生物标志物。因此,我们旨在从代谢组学的角度确定光疗时机的新预测因子.
UNASSIGNED:入院当天共招募12例新生儿高胆红素血症新生儿。根据光疗治疗的时间长短,将婴儿分为短期(<30小时)光疗组和长期(≥30小时)光疗组。然后使用非靶向代谢组学策略探索血清样品中的代谢物。
未经批准:总共,在短期和长期光疗组之间鉴定出1,073个显着不同的代谢物中的59个,包括18种上调和41种下调的代谢物。代谢组学分析结果表明,差异表达的代谢产物富含甘油磷脂代谢,与胆红素的排泄密切相关。此外,京都基因和基因组百科全书(KEGG)途径分析表明,代谢物还富含α-亚麻酸代谢和脂肪酸伸长。Spearman相关层次聚类分析表明,9种代谢物与光疗持续时间呈负相关。代谢物,特别是磷脂酰乙醇胺(PE)(22:1(13Z)/15:0),磷脂酰胆碱(PC)(18:1(9Z)/18:1(9Z)),磷脂酰丝氨酸(PS)(22:0/15:0),5,6-二氢尿苷,和PE(MonoMe(11,3)/MonoMe(13,5)),光疗持续时间的可预测性[曲线下面积(AUC):1;95%置信区间(CI):1-1]比总血清总胆红素和直接胆红素(AUC:0.806;95%CI:0.55-1),如接收器工作特性分析所揭示的。
UNASSIGNED:我们的研究发现,差异代谢产物与新生儿黄疸的持续时间有关,甘油磷脂代谢可能在这一生物学过程中起作用。此外,代谢物,如PE(22:1(13Z)/15:0),PC(18:1(9Z)/18:1(9Z)),PS(22:0/15:0),5,6-二氢尿苷,和PE(MonoMe(11,3)/MonoMe(13,5))可用作新生儿高胆红素血症光疗持续时间的预测因子,并有助于决策。
UNASSIGNED: Phototherapy is a recommended method for the treatment of neonatal hyperbilirubinemia. However, biomarkers for predicting the more effective duration of phototherapy prior to treatment are lacking. Therefore, we aimed to determine novel predictors for the timing of phototherapy from the perspective of metabolomics.
UNASSIGNED: A total of 12 newborns with neonatal hyperbilirubinemia were recruited on the day of admission. The infants were divided into a short-duration (<30 hours) phototherapy group and a long-duration (≥30 hours) phototherapy group based on the length of phototherapy treatment. Metabolites in serum samples were then explored using an untargeted metabolomics strategy.
UNASSIGNED: In total, 59 of 1,073 significantly different metabolites were identified between the short-duration and long-duration phototherapy groups, including 18 upregulated and 41 downregulated metabolites. The results of metabolomic analysis showed that the differentially expressed metabolites were enriched in glycerophospholipid metabolism, which is closely associated with the excretion of bilirubin. Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the metabolites were also enriched in alpha-Linolenic acid metabolism and fatty acid elongation. Spearman correlation hierarchical clustering analysis demonstrated that 9 metabolites were negatively correlated with the duration of phototherapy. Metabolites, especially phosphatidylethanolamine (PE) (22:1(13Z)/15:0), phosphatidylcholine (PC) (18:1(9Z)/18:1(9Z)), phosphatidylserine (PS) (22:0/15:0), 5,6-dihydrouridine, and PE (MonoMe(11,3)/MonoMe(13,5)), had better predictability for the duration of phototherapy [area under curve (AUC): 1; 95% confidence interval (CI): 1-1] than total serum total bilirubin and direct bilirubin (AUC: 0.806; 95% CI: 0.55-1), as revealed by receiver operating characteristic analysis.
UNASSIGNED: Our research found that the differential metabolites were associated with the duration of neonatal jaundice and that glycerophospholipid metabolism might have played a role in this biological process. Moreover, metabolites such as PE (22:1(13Z)/15:0), PC (18:1(9Z)/18:1(9Z)), PS (22:0/15:0), 5,6-dihydrouridine, and PE (MonoMe(11,3)/MonoMe(13,5)) could be used as predictors for phototherapy duration in neonatal hyperbilirubinemia and assist with decision-making.