■小肠运动改变与肠易激综合征之间的相关性没有得到很好的评价。本研究旨在评估具有束缚应激的青少年肠易激综合征大鼠模型中的小肠和结肠过境,并确定小肠动力在肠易激综合征病理生理中的作用。
■利用约束应激来制备青少年肠易激综合征大鼠模型,对其进行临床体征评估,包括大便次数和腹泻。还评估了小肠运动和转运率。
■编码促肾上腺皮质激素释放激素的mRNA数量,肥大细胞,使用实时聚合酶链反应定量5-羟色胺(5-羟色胺)受体3a;使用免疫染色评估5-羟色胺的表达。
■限制压力显着增加了粪便颗粒输出的数量,大便含水量,青少年肠易激综合征大鼠模型的小肠运动。实时聚合酶链反应结果没有差异;然而,免疫染色分析表明,在青少年肠易激综合征大鼠模型中,小肠中5-羟色胺的表达显着增加。
■在约束应激的青少年肠易激综合征大鼠模型中,我们观察到小肠和结肠运动增加。在小肠里,远端部分的5-羟色胺分泌增强可能与增加小肠运动有关。虽然目前的研究集中在5-羟色胺,进一步研究调节肠蠕动的其他因素可能会导致建立更有效的治疗青少年肠易激综合征的方法。
UNASSIGNED: The correlation between altered small intestinal motility and irritable bowel syndrome is not well evaluated. This study aimed to assess the small intestinal and colonic transits in an adolescent irritable bowel syndrome rat model with restraint stress and determine the role of small intestinal motility in the irritable bowel syndrome pathophysiology.
UNASSIGNED: Restraint stress was utilized to prepare adolescent irritable bowel syndrome rat models that were evaluated for clinical signs, including stool frequency and diarrhea. The small intestinal motility and transit rate were also evaluated.
UNASSIGNED: The amounts of mRNA encoding corticotropin-releasing hormone, mast cell, and serotonin (5-Hydroxytryptamine) receptor 3a were quantified using real-time polymerase chain reaction; the 5-Hydroxytryptamine expression was evaluated using immunostaining.
UNASSIGNED: Restraint stress significantly increased the number of fecal pellet outputs, stool water content, and small intestinal motility in the adolescent irritable bowel syndrome rat models. There was no difference in real-time polymerase chain reaction results; however, immunostaining analysis revealed that 5-Hydroxytryptamine expression in the small intestine was significantly increased in the adolescent irritable bowel syndrome rat models.
UNASSIGNED: In the rat model of adolescent irritable bowel syndrome with restraint stress, we observed an increase in small intestinal and colonic motility. In the small intestine, enhanced 5-Hydroxytryptamine secretion in the distal portion may be involved in increasing the small intestinal motility. Although the present study focused on 5-Hydroxytryptamine, further investigation of other factors that regulate intestinal peristalsis may lead to the establishment of more effective treatment methods for adolescent irritable bowel syndrome.