近年来,海洋哺乳动物的传染病有所增加,包括布鲁氏菌病,麻疹病毒感染,疱疹病毒,和痘病毒。几种血清学诊断方法,包括酶联免疫吸附测定,免疫荧光测定(ELISA),和西方印迹,已被用于检测海洋哺乳动物中针对病原体的抗体。然而,用于检测海洋哺乳动物抗体的商业二级抗体的选择有限;因此,蛋白质A的使用,G,或嵌合蛋白AG可以提供合适的替代方案。这项研究旨在评估蛋白质A的使用,G,和嵌合蛋白AG检测海洋哺乳动物免疫球蛋白。目前,没有关于蛋白质A的使用的比较研究,G,和嵌合蛋白AG用于检测海洋哺乳动物的免疫球蛋白。在这项研究中,我们使用了十种针脚(贝加尔湖海豹,加州海狮,海豹港,北方海豹,环状印章,南美海豹,南美海狮,斑点海豹,斯特勒海狮,和海象)和五种鲸类(白鲸,宽吻海豚,港口海豚,虎鲸,和太平洋白边海豚),并比较与蛋白质A的结合能力,G,或嵌合蛋白AG通过ELISA。结果表明,与蛋白G相比,来自pinnipped和鲸目动物物种的免疫球蛋白对蛋白A的反应更强烈。pinnipes和鲸目动物的免疫球蛋白对嵌合蛋白AG具有很强的结合能力。这些结果表明,蛋白质A,G,和嵌合蛋白AG将用于进一步开发血清学测定。
In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds\' species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays.